The Expression Pattern Of RNA Helicase Mvh In Mouse Brain During Postnatal Development

Vasa was first identified in the reproductive system of Drosophila melanogaster,which was essential for development of germ cells.The mouse Vasa homologous(Mvh)was also specifically expressed in mouse germline and was thought to be a specific marker to study the division,differentiation,migration of germ cells.As a class of highly specialized cells,neurons are structurally composed of axons and dendrites.In axons,local translation provides a precise and prompt mechanism for spatiotemporal expression of mRNA which takes effect in axon regeneration or growth,growth cone pathfinding and retrograde signal which is mediated by RNA binding proteins.RNA-binding proteins bind to target mRNAs and assemble into transport particles,then it transported along the microtubules to axons where mRNAs translated into proteins.As a member of RNA helicase family,Mvh was also proved to be involved in RNA metabolism,including RNA binding and RNA slicing.Recent studies have shown that Mvh interacts with multiple mRNAs in mouse testis,including Prmt5,Dicer.As a protein arginine methyltransferase,prmt5 plays an important role in cell differentiation of neural stem and embryonic development.Dicer is mainly responsible for the generation of microRNAs,which are widely involved in various biological processes in cells.Taken together,it suggested that Mvh may play an important role in the nervous system,especially in local translation.In this study,our results show that Mvh is widely expressed in the cerebral cortex,olfactory bulb and cerebellum in mouse brain during postnatal development.In cerebral cortex,the expression of Mvh increases firstly(P8-P21)and then decreased(P30-P60)with teh development of mouse,and it is mainly distributed in the nerve fiber segment.In olfactory bulb,the expression of Mvh increased gradually(P7-P14)with the development of mouse,and then gradually decreased(P21-P60),it mainly distributed in the external plexiform layer and granular cell layer.In cerebellum,Mvh is specifically expressed in purkinje cells(P30-P60)and the signal is significantly reduced in Reeler mouse whose physical balance is disrupted.It suggested that there may be a functional link between Mvh and reelin in cerebellum.Intriguingly,Mvh positive nerve fibers formed some special nest-like structure in cerebral cortex.In addition,although we used specific nerve cells and nerve fibers markers,we did not identify the type of Mvh-positive cells and fibers in the cortex and olfactory bulbs.In summary,we are the first studied the expression pattern of Mvh in the mouse brain during postnatal development,which laid the theoretical foundation for the further study of the function of Mvh in nervous system and the mechanism of local translation of axon.