| Adipose tissue with storage of energy,regulation of secretion,immunity,repair trauma and other aspects of the function.Altay sheep's tail fat accounted for 17% of body weight.At present,a comparative study of sheep in different adipose tissue ultrastructure and function is rarely reported.In this study,we will study the cell structure,fatty acid composition and function of Altay sheep's tail fat,and explore the multidimensional value of the development of the sheep tail fat.1.Ultra-microstructure analysis of fat cells of Altay sheep's tail fat,sheep perirenal fat and bovine perirenal fatty.In this study,six 15-month-old adult malay large tail sheep and female Holstein cows were randomly divided into two groups: the fresh sheep fat,the sheep's perirenal fat,and the fresh bovine perirenal fat.The ultrastructures of the cells were observed and compared by transmission electron microscopy and paraffin section.The results showed that under TEM in Altay sheep tail fat fat cell membrane outside of a special "hair" structure,are distributed around the black punctate granules;two kinds of perirenal fat cells are clearly visible,shapes of fat droplets,and a large number of different arrangement of endoplasmic reticulum,mitochondrial structure.The study shows that Altay sheep tail fat fat cells and perirenal fat and perirenal fat week cow fat cells showed obvious differences in ultrastructure.2.Altay fat tailed sheep tail fat,perirenal fat and bovine perirenal fatty acid composition analysis.The purpose of this study was to study the fatty acid composition of the big tail sheep tail fat from Altay and to be compared with the perirenal fat and bovine perirenal fat,and to study the fatty acid composition ratio and its role.The GC-MS was used to analyze the fatty acid composition of the big tail Sheep's tail fat and perirenal fat and the bovine perirenal fat by 37 kinds of fatty acid standards.The results showed that the content of saturated fatty acids was the highest(72.7%),and the content of polyunsaturated fatty acids in bovine perirenal fat was the highest(14.92%).Three kinds of fat contain a lot of lauric acid,myristic acid,palmitic acid,In the sheep's tail fat,The content of lauric acid was 20.69% of the total fatty acids,In the sheep's perirenal fat,Palmitic acid content of the total fatty acids 24.06%.Lauric acid,myristic oleic acid,palmitoleic acid and other contents in the big tail Sheep's tail fatty acid were significantly lower than perirenal fat(P < 0.01);Lauric acid,pentadecanoic acid,palmitoleic acid,ginkgolic acid,linoleic acid and tricosanoic acid in the big tail Sheep's tail fatty acid were significantly higher than bovine perirenal fat(P <0.01);The results showed that the fatty acids in the three fats had their own characteristics,and the content of fatty acids was also very different.3.Repair effect of Aletai sheep tail fat on damaged skin.This study was to investigate whether the large tail sheep fat of the Aletai had a repair effect on the damaged skin and compared it with the bovine fat and mupirocin ointment.A total of 40 Kunming mice weighing between 28 and 32 g were divided into two groups through the construction of mouse skin damaged model.The study found that mice skin wound healing well and the female's wound healing ability is stronger than the male mice,the wound healing rate of the female and male mice were Sheep tail fat > Bovine fat > Mupirocin ointment > Control group and Sheep tail fat > Mupirocin ointment > Bovine fat > Control group.Skin histopathological results showed that the number and arrangement of adipocytes,collagen fibers and other cells in the wound repair tissues were different compared with those of the blank control group.Studies have shown that sheep fat has the ability to repair wounds and helps to promote damage to the skin wound healing. |
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