Effects Of Orthopedics And Rootstock On Sugarandacid Metabolism And Organic Acid-related Gene Expression In Grape Fruits

Organic acids and sugars play a key role in the quality of grapes and the flavor of wines.Sugar and organic acids are particularly important.In production,people often use different cultivation methods and rootstock grafting to obtain high quality wine grapes,thereby improving the quality of wine.In this experiment,'Cabernet Sauvignon'and'Chardonnay' grapes from Yiyuan Vineyard in Jinzhong,Shanxi Province were used as experimental materials to study the sugar and organic acids in berries and their related metabolic enzymes,which provided a strong theoretical basis for improving the quality of local wine grapes.The main results are as follows:1.This experiment by studying the different tree 'cabernet sauvignon fruit sugar metabolism related enzyme activity and its content changes,the results show that: in the whole growing season,different tree'cabernet sauvignon berries of SS,SPS and NI activity difference is far less than the difference of acid invertase AI,on the growth development period,plant glyph fruit of glucose,fructose and reducing sugar content were significantly higher than the fan.2.In this experiment,the acid metabolism-related enzyme activities and their content changes in different tree 'cabernet sauvignon' fruits were studied.The results showed that the activities of nad-mdh and nadp-me were related to the content of malic acid.25 d after flowering,it was found that the activities of nad-mdh and nadp-me enzymes in the plant-shaped fruits were lower than those in the fan-shaped fruits,and the synthesis and degradation of malic acid in the fruits were consistent.At this time,the contents of malic acid in the fruits of the two tree types were basically the same.However,with the growth and development of fruits,the content of nad-mdh in fan-shaped fruits was 50.95% higher than that in fan-shaped fruits 65 days after flowering,while nadp-me was 48.76% lower than that in fan-shaped fruits.The change of nad-mdh was large,and the content of malic acid in fan-shaped fruits 65 days after flowering was higher than that in fan-shaped fruits.The enzyme activity of PEPC was negatively correlated with the content of malic acid,and the content of malic acid and titration acid in the whole fruitdevelopment process was lower than that in the fan-shaped grape.The content of tartaric acid in the two kinds of tree fruits was basically the same,and the content of citric acid in different trees was also different.3.By studying the changes of NAD-MDH,NADP-ME and PEPC gene expression levels in different tree 'cabernet sauvignon' fruits,this study found that NAD-MDH and PEPC were positively correlated with the content of malic acid,while NADP-ME and PEPC were negatively correlated with the content of malic acid.NAD-MDH and NAD-MDH enzyme changes were consistent,but NADP-ME and PEPC did not correspond to the enzyme activities of NADP-ME and PEPC in fruits.4.In this study,the sugar metabolism-related enzyme activities and content changes of cabernet sauvignon fruits on different stocks were studied.The results showed that the reducing sugar content of cabernet sauvignon fruits grafted on SO4 stocks was significantly lower than that of cabernet sauvignon root trees during the whole growth and development process.At the early stage of fruit development,the change rule of AI enzyme activity was consistent with that of reducing sugar content in fruits of different stocks,but at 65 days after flowering,AI activity was contrary to that of reducing sugar content in fruits of different stocks.5.In this study,acid metabolism-related enzyme activity and its content in cabernet sauvignon fruit of different stocks were studied.The results showed that SO4 rootstock significantly increased the content of titratable acid and malic acid in fruit.Rootstock had no effect on tartaric acid content of 'cabernet sauvignon' fruit,and SO4 rootstock also had effect on citric acid content of 'cabernet sauvignon' fruit,but the difference was not significant.25 d,65 d after flowering,in SO4 'cabernet sauvignon fruit on the root of NAD-MDH enzyme activity is higher than the' cabernet sauvignon 'since the root tree,NAD-MDH and malic acid synthesis,25 d-65-d after flowering,NADP in control fruits-ME activity is higher than the SO4 root stock but no significant difference,while the SO4 root stock the malic acid content of fruit was also significantly higher than that of control,NADP-ME promote the degradation of malic acid.6.In this experiment,changes in the expression levels of NAD-MDH,NADP-ME and PEPC genes in the fruit of 'cabernet sauvignon' were studied.It was found that NAD-MDH,NADP-ME,and PEPC were related to the synthesis of malic acid.NAD-MDH and NAD-MDH enzyme activities were consistent,while NADP-ME and PEPC were not corresponding to NADP-ME and PEPC enzyme activities.7.This experiment by studying the different tree 'chardonnay' fruit sugar metabolism related enzymeactivity and its content changes,the results show that in the late' chardonnay fruit growth(45 d-65 d)after flowering,the fan in the fruit soluble total sugar,reducing sugar,glucose and fructose were higher than factory glyph,AI enzyme activity in the fruit in the 85 d after flowering and fruit sugar content,but in the65 d after flowering,fruit sugar content and its enzyme activity are not corresponding.8.This experiment by studying the different tree 'chardonnay' fruit sugar metabolism related enzyme activity and its content changes,the results show that the factory glyph significantly improve fruit of malic acid,citric acid,propionic acid and the content of ascorbic acid in NAD-MDH,NADP-ME and PEPC and malic acid content change study,NAD-MDH and malic acid content were positively correlated,and negatively correlated with NADP-ME.