| Ziziphus jujuba Mill.'Huping zao' is rich in nutrition and ranks first in all nutrients of the fruits,not only edible but also extremely high medicinal value.In this paper,compared with the control group of clear water,the concentration of the best Ca that can reduce the cracking rate of Z.jujuba Mill.'Huping zao' was selected through 6 concentrations of three Ca.In addition,the content of protein in the Z.jujuba Mill.'Huping zao' was closely related to the changes of the components,and the number and depth of proteins in different parts of Z.jujuba Mill.'Huping zao' corresponded to the level of protein in the electrophoretogram.Through various treatments of different Ca,proteomics research methods are used to provide theoretical basis for the prevention and treatment of the fruit cracking of Z.jujuba Mill.'Huping zao' from the protein level.The conclusions are as follows:(1)Compared with the control group,3 kinds of Ca can reduce the fruit cracking rate of Z.jujuba Mill.'Huping zao' after spraying.After 500 times liquid of CaCl2 spraying,the rate of cracking was 11.34%decreased the most obvious,which was 30.11%lower than the control.It is found out that the lower to higher cracking rate were 500 times liquid CaCl2,300 times liquid CaCl2,500 times liquid Gaizhongbao,1000 times liquid EDTA,1000 times liquid Gaizhongbao,1500 times liquid EDTA.(2)Compared with the control group,the protein content of Z.jujuba Mill.'Huping zao' leaves,peel and pulp were significantly increased with different types of Ca,and the difference were significant.The protein content in leaves under the influence of 500 times liquid of CaCl2 was the highest 3.58mg·g-1,which was 1.03mg·g-1 higher than the control;the highest protein content in peel was under the influence of 300 times liquid of CaCl2 was 4.58mg g-1,2.39mg·g-1 higher than the control;the protein content in the pulp under the influence of 500 times liquid CaCl2 was 4.58mg·g-1,which was 2.53mg·g-1 higher than the control.(3)In the electrophoretic map,compared with the control,the leaves,peel,and pulp protein bands of Z.jujuba Mill.'Huping zao' after spraying with different Ca formulations were significantly increased,and there were significant differences.Leaves in 5 kinds of protein components are mainly concentrated in 25kDa?10kDa low molecular weight,molecular weight in the 20kDa?56kDa area there are several identify the not clear of protein bands,and the protein bands appear under the influence of CaCl2 and 500 times liquid Gaizhongbao.not much differences.The 13 protein components in the peel were mainly concentrated in the low molecular weight region of lOkDa?25kDa and the middle molecular weight region of 45kDa?66kDa,with the largest number of protein bands under the influence of 300 times liquid CaCl2.The 13 proteins in the pulp were distributed in the middle and low molecular weight region of 10kDa?48kDa,and the number of protein bands was the most under the influence of 300 times liquid CaCl2.The quantity,clarity,and color depth of the protein subunits in the SDS-PAGE electrophoresis map directly reflect the protein content.The higher the protein composition and the darker the protein subunit,the higher the protein content.Z.jujuba Mill.'Huping zao' leaves,peel,pulp protein content and the corresponding SDS-PAGE electrophoresis can be concluded that how much protein in the electrophoresis bands and depth and protein content of the corresponding conclusion.(4)The fruit cracking rate of Z.jujuba Mill.'Huping zao' was significantly negatively correlated with the protein content of peel and pulp.The correlation between pulp and leaf did not reach significant levels.There was a very significant positive correlation between peel and pulp.The leaf and peel did not reach significant levels. |
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