Study Of Embryonic Development,larval Oxygen Consumption Rate And Tissue Pathology Caused By Hypoxia Of Myxocyprinus Asiaticus

The the mullet(Myxocyprinus asiaticus)as the experimental material by stereo microscope observation in vivo and tissue observation of two methods,in Tianjin for new aquatic breeding field may 2015 hatchery production of the rouge fish embryos until the film 14 days of larval development was observed and the statistics in water temperature 21.0~23.0 DEG C under total development time is longer,about 119 H40 min and effective accumulated temperature for 1743.59 deg.c,the average fertility rate for 68.51%.Rouge fish eggs telolecithal egg,sinking,micro viscous,embryonic development after cleavage,blastula,gastrula stage,neural embryo stage,organ differentiation and hatching in six stages.The newly hatched larvae yolk sac shaped,in the film tenth days after absorption.The heart structure was seen in second days in the pericardial membrane,the heart gradually increased,blood color gradually deepened.In the film second branchial arch pulley outline is clear,fourth days showed 4 of the branchial arch,gill filaments and gradually increased the number of.First days of the film can see the crystal,retina and pigment,and then the retina,pigment layer gradually thickened,to the film 8~14 days,the eyes of the posterior chamber is more obvious,there is no anterior chamber.The inner ear distribution in the medulla at the left and right sides,there are listening to the board,visible sensory hair and round otolith.The brain has 5 parts for the differentiation of the telencephalon,diencephalon,mesencephalon,cerebellum and medulla in brain membranes before and after gradually increased.Mouth is composed of a lower gradually transferred to the sub lower,intestinal digestive tract in a coiled appears in front of the film,sublevel open.In the film the 4 days of digestive tract is completely open.The kidney is the main organ of excretion,the first day of the film can be seen,and then gradually increased.In the film second days inflatable bladder,until the film 14 days for room 1.The distribution of astrocytes in the head,back and abdomen.The oxygen consumption rates(OCR)and suffocation points(SP)were investigated for three-months mullet at18,21,24,27and30? by closed means of sealed-still water.The results showed that the OCR of mullet was extremely significant in different groups(P<0.01).The OCR of mullet increased with the rising of temperature,The relationship of them could be described as y=0.012x-0.087(n=10,P<0.01,R2=0.7603).The lethal dissolved oxygen content of mullet showed no significant difference among groups(P>0.05).The determination of death induced point in March after the age of juvenile Myxocyprinus asiaticus asphyxia,fixed with Bouin 's solution,the dehydration of tert butyl alcohol,paraffin,H staining,E,neutral balata,histopathological observation,the death from lack of juvenile shows visible fish brain,gill,gill artery artery,heart,hepatopancreas,spleen,kidney in arterial hyperemia,venous congestion,gill,heart,kidney appeared hemosiderin,gill lamellae respiratory epithelial cells stripping,liver cells,hydropicdegeneration ballooning,renal tubular epithelial cells hyalinization and body organs hypoxia leads to organ metabolic abnormalities,which showed that hypoxia Myxocyprinus occurred mainly in blood circulation disorders caused by death.