Attraction Effect Of Host Plant Volatiles On Lygus Pratensis And Its Field Effect Evaluation

Lygus pratensis is the dominant species of blind mites in cotton fields in Xinjiang.In recent years,the population of Lygus pratensis is increasing as well as damages caused by them.This study intends to further explore the active constituents which attract Lygus pratensis in plant volatiles.We tested the standard compounds and their different compounds in 16 plant volatiles based on the research carried by Sun et al.The optimal concentration and ratio of the compounds with a strong attraction to Lygus pratensis were selected.The field trapping test was also carried out.The results are as follows:1.The host plant volatiles influenced the olfactory behavior of male and female adults.The Y-type olfactometer was used to determine the olfactory behavioral response of the male and female adults to the 16 plant volatiles.The results showed that the responses of the female larvae to the four concentrations of sixteen volatile substances had a simila r inverted V-shape trend.Volatile concentration up to 10^-3?v/v?and?-pinene concentration up to 10^-4?v/v?can trigger Statistically significant?P<0.01?olfactory behavioral responses of pasture vaccinia were triggered when the concentration of heptacosane,n-nonadecane,n-nonanal,heptadecane,3-vinyl-1-ol,and dodecane reached 10^-3?v/v?and the concentration of?-pinene reached 10^-4?v/v?.?-pinene with a concentration of 10^-4?v/v?had the highest rate?63.33%?of attraction among all tested volatiles.The effect of volatiles on female Lygus pratensis adults is significantly higher than male adults.The mixture of Acetone 10^-3:SS-decene 10^-4:3-hexene-1-alcohol 10^-3?v/v?with the proportion of 1:5:1 respectively had the highest attraction rate which was 66.67%.2.The results of the electrophysiological reaction of the Lygus pratensis on the volatiles of 16 host plants showed that the electrophysiological responses of the Lygus pratensis were different among different volatiles.The EAG responses of the females were higher than those of the males.?-pinene had the strongest EAG reaction on Lygus pratensis while ionone had the weakest.3.The field trapping experiments of host plant volatiles on Lygus pratensis showed that the single agents with the best trapping effect were heptadecane,acetaldehyde,and?-pinene.Among all the tested mixtures,the formulae with ideal trapping efficacies for adults are:Sunflower aldehyde 10^-3?v/v?and 3-hexen-1-ol 10^-3?v/v?with a proportion of 1:5;nonadecane10^-3?v/v?,SS-pinene 10^-4?v/v?,and twelve Alkane 10^-3?v/v?with a proportion of 1:1:5;sunflower aldehyde 10-3?v/v?,SS-pinene 10^-4?v/v?,3-hexen-1-ol 10^-3?v/v?with a proportion of 1:5:1.