Effect Of Fermented Biological Feed On Growth Performance,Digestive Enzyme And Immune Enzyme Activity Of Macrobrachium Rosenbergii

In order to explore the effect of biological fermentation feed on growth and immunity of Macrobrachium rosenbergii,the study detected growth performance,digestive enzyme and immune enzyme activity of M.rosenbergii after feeding biological fermentation.Fermented biological feed is new-style feed,whose products included peptide,fatty acid,digestive enzyme,amino acid,vitamin and mineral.The microbes could eliminate anti-nutritional factors,and were also advantageous to the absorption of animal body nutrition,palatability and feed conversion ratio of feed.The feed after fermentation had some beneficial bacteria,which could be dominant bacteria and could produce a variety of organic acids,reducing intestinal pH value,and inhibiting the growth of harmful bacteria.In the study,the M.rosenbergii with an average body weight of(0.02±0.001)g were randomly divided into 3 groups with 3 replicates per group and 2400 shrimp per replicate.Finally,they were fed different proportion of fermented biological feed for 42 days.The four treatment groups were as follow: group A was fed 100% feeding basic diet,group B was fed 90% feeding basic diet with 10% microorganism starter cultures,group C was fed 80% feeding basic diet with 20% microorganism starter cultures,group D was fed 70% feeding basic diet with 30% microorganism starter cultures.Tanking samples of M.rosenbergii were measured data of the growth performance,muscle nutrition indicators,digestive enzyme and immune enzyme activity.The results showed as follows:1.Fermented biological feed could increase weight gain.The weight gain of M.rosenbergii from group C was remarkably higher than those of M.rosenbergii from group A(P<0.01),increased 7.42% compare with group A.Similarly.The weight gain of M.rosenbergii from group D was significantly higher than those of M.rosenbergii from group A(P<0.05),increased 4.61% compare with group A.2.Fermented biological feed had no significant in muscle nutrition composition and content of amino acids of M.rosenbergii(P>0.05).The group C was the most obvious,its content of crude protein,total amino acid,flavor amino acid and essential amino acid were 19.00%,18.04%,6.74% and 8.27%.The muscle amino profile of M.rosenbergii had 17 amino acids,which were in accordance with the FAD/WHO.3.Fermented biological feed could improve digestive enzyme activity of M.rosenbergii.With the increasing amount of microorganism starter cultures additives,trypsin and cellulose activity of M.rosenbergii saw a trend from rising to downward,the group C was the most obvious,its content of trypsin and cellulose activity were 145.07U/mg prot and 3.06 U/mg prot,the trypsin and cellulose activity of M.rosenbergii from group C was remarkably higher than those of M.rosenbergii from group A(P<0.01),respectively increased 45.52% and 18.60% compare with group A.On the other hand,amylase activity presents an increasing trend and the group D was the highest,it was significant different from the other three groups(P<0.01).4.Fermented biological feed could improve immune enzyme activity of M.rosenbergii.The superoxide dismutase and glutamic-oxalacetic transaminease activity of M.rosenbergii from group C were notably higher than those of M.rosenbergii from group A(P<0.05),respectively increased 7.09 % and 17.09% compare with group A.In addition,the superoxide dismutase activity of M.rosenbergii from group D was remarkably higher than those of M.rosenbergii from group A(P<0.01),increased 19.31% compare with group A.In the present study,the best range of microorganism starter cultures additives is 20% to 30%,which can improve weight gain,amylase,cellulose,superoxide dismutase and glutamic-oxalacetic transaminease activity of M.rosenbergii.