| Light which plays an important role in regulating physiological function of animal is one of the most important factors for animal developing.The light is the key impact of the animals' fur growth.Appropriate lighting can not merely improve the metabolism and appetite,increase animal fur quality and breeding ability,and reduce mortality.Previous studies have found that different wavelength of light can effect growth and development of rabbits at a certain extent.This study is conducted to explore the effect of different LED light on the rabbit growth and fur development and to provid a date reference for rabbits breeding.By using the white light,red light,green light,blue light and black light to shine rabbits respectively,we evaluated the production performance and the body's antioxidant capacity,detected the rabbit hair quality,and observed the change of hair follicle structure.Experiment 1.The effect of different LED light on the hair quality of long-haired rabbitFifty,sixty day old rabbits with health and similar weight were randomly divided into 5 groups,10 pear each group,trimed and continuously raised 72 days on LED light.At the end of the experiment,collecting the hair samples of the shoulder,back and abdomen of rabbits in each group.Respectively measuring the hair length,the rate of coarse hair,diameter,strength and moisture regain,analyzing the effect of different LED light on hair quality.The results showed that:The hair length on the shoulder and back of rabbits treated by the red light were the longest,respectively 57.84 mm and 54.53 mm,and respectively 26.23%(P<0.05)and 14.78%(P>0.05)longer than that of the white group.On the rabbit abdomen,the longest hair,45.11 mm and the shortest hair,34.32 mm,was respectively in the white light and the black light groups.There were no significant differences among groups(P>0.05).The highest rate of coarse hair was 13.31%in the green light group,the lowest rate of coarse hair was 3.81%in the red light group,and there was significant difference in rate of coarse hair between this two groups(P<0.05).The maximum diameter of coarse was 49.93 ?m in the green light group,41.01 ?m in the black light group,which smaller than that in the green light group.The minimum diameter of coarse hair was 28.70 ?m in the white light group and significantly less than that of the green and black light groups(P<0.05).The maximum hair strength,17.87 cN and the minimum,14.04 cN was respectively in the black and the white light group,there were no significant differences among groups(P>0.05).The higher moisture regain in the white light and the red light group was respectively 14.21%and 13.73%.But there were no significant differences among groups(p>0.05).Experiment 2.The effect of different LED light on the weight change,the skin hair follicle structure and the skin mRNA of Wnt10b of long-haired rabbitFeeding process the same as experiment one,weighing four times during breeding,statistical the changes of average daily gain under five different monochromatic lights.The results showed that:The red light,the green light and the blue light,the white light were respectively in the early,middle and later promoted the growth of the rabbits in the experiment.At the end of test,selecting five rabbits with similar weight in each group after collected the hair.A piece of back skin in each rabbit were cut to tile the perforate board and fixed in Bourne's solution for 8-24 h,then dehydrated,made transparent,embedded,sliced into 6 microns.After HE staining,the changes of the skin follicle structure were watched under the light microscope.Results show that:The capsule groups under the red light were the most,and most of them were secondary follicle,primary follicles rarely,hair follicle distributed uniform.The capsule groups structure under white light,blue light and black light were in normal level.The capsule groups under green light at least,and the hair follicle distributed not uniform.Choosing five similar weight rabbits from each group at the end of the experiment.After taking the skin at the same part of back,through RT-PCR technique,statistic the expression of Wnt10b.Results show that:The highest WntlOb expression was under the black lights(0.05<P<0.01),respectively higher 27.08%,33.50%,39.17%and 49.38%than the other four lights,which was white light,green light,red light and blue light.There was no significant difference between each groups(P>0.05).Experiment 3.The effect of different LED light on the trace element contents in whole blood and hair of long-haired rabbitFeeding process the same as experiment one,choosing five rabbits withsimilar weight from each group at the end of the experiment.Blood samples were collected from the heart of rabbits and hair samples were collected on the side of scapular.Measuring the content of trace elements.The results showed that:White light was beneficial to the Mg and Fe absorption in blood,good for B,Cu,Mn and P absorption of the hair.Red light was conducive to the absorption of Cu,Zn and Cd in blood,conducive to the absorption of A1 in the hair.Green light was conducive to the absorption of Mo and I in the hair.Blue light was conducive to the absorption of Ca and Pb in the blood,good for Mg,Fe,Pd and Cd absorption of the blood.Black light was conducive to the absorption of Zn,Ca,and Cr in the hair.Experiment 4.The effect of different LDE light on the antioxidant capacity of the long-haird rabbitFeeding process the same as experiment one,choosing five rabbits withsimilar weight from each group at the end of the experiment.Blood samples were collected from the heart of rabbits and separated plasma in-20 ?,took the skin tissue at the same time.Determination of antioxidant index respectively:The GPx,SOD activity and T-AOC,and the units of MDA.The results showed that:The antioxidant capacity of skin and plasma in different performance under different monochromatic light.Green light increased T-SOD vitality of the skin,and blue light increased T-SOD vitality of the plasma.White light and black light increased GSH-Px levels.The highest MDA content in skin was under the green light and the highest MDA content in blood was under the blue light.The T-AOC of the skin under the white light and black light were bigger than other groups,The T-AOC of the blood under red light,green light and blue light were bigger than other groups.Conclusion:Red light can increase the hair length,the rate of fuzz hair,moisture regain,and reduce the hair diameter,which consistent with the hair follicle structure,increased the number of secondary follicle.Increased Cu and Zn content in the blood and the A1 content of hair;Green light increased the coarse rate and the coarse diameter,which consistent with hair follicle structure.Red light,green light and blue light,white light,pectively promoted the rabbits growth at early,middle and late of the experiment.The T-AOC activity under the white light and black light in the skin were the larger than the other groups,and the T-AOC activity under the red light,green light and blue light in the blood were the larger than the other groups.The WntlOb genes relative to express under the black light was the largest in the late test. |
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