Transcriptomic And Proteomic Analysis Of Flubendiamide Resistance In Plutella Xylostella L.

Diamondback moth?Plutella xylostella L.?belongs to Lepidoptera Plutellidae which is one of cosmopolitan pests of cruciferous vegetable crops and causes huge economic losses each year.At present,the diamondback moth has developed resistance to almost all chemical insecticides.Since the advent of flubendiamide,the resistance of diamondback moth to this insecticide has developed rapidly due to irrational drug use.In order to investigate the resistance mechanism of Plutella xylostella to flubendiamide,the RNA-seq analysis method and iTRAQ analysis method were used in this study to sequencing and analysing of a relative sensitive strain?S?collected in Plant Protection Experimental Station of the Southern Campus of Shandong Agricultural University,a resistance strain to flubendiamide?Rh?,a field resistant strain R_b collected by Guangzhou Baiyun Airport and a flubendiamide resistant strains treated by quercetin(Q_Rh).This research provides strong support for revealing the biological characteristics of the resistant Plutella xylostella,the difference in the transcriptional and protein levels of the resistance and sensitive strains of the diamondback moth and resistance mechanism of the diamondback moth to the flubendiamide.The results of the study are as follows:1.On the basis of previous studies,the resistant strain of flubendiamide in the laboratory for a long time maintenancing was reversely selected to obtain homozygous GAG genotype of 4946 locus.Further flubendiamide selective pressure was carried out to obtain a resistance strain?Rh?with resistance multiple of 1889.82 times.The resistance of the field-resistant Plutella xylostella population?Rb?to flubendiamide was measured as 1250.97-fold.2.Differentially genes/proteins:S-VS-Rh comparison group obtained 1019 differentially expressed genes and 168 differentially expressed proteins;S-VS-Rb comparison group obtained 1962 differentially expressed genes and 366 differentially expressed proteins;Rh-VS-Rb comparison group obtained 2417 differentially expressed genes and 448differentially expressed proteins;there were 597 differentially expressed genes and 191differentially expressed proteins among Q_Rh-VS-Rh comparison group.3.GO enrichment analysis and Pathway enrichment analysis of differentially expressed genes/proteins revealed that differentially expressed genes/proteins are mainly involved in metabolic processes,catalytic activity,binding factors,oxidation and other functions and processes.4.Screened resistance-associated differentially expressed genes/proteins of target receptors and detoxification enzyme:there were 10 differentially expressed genes and 1differentially expressed proteins of RyR;51 differentially expressed genes and 16differentially expressed proteins of CYP,15 differentially expressed genes and 15differentially expressed proteins of GST,12 differentially expressed genes of CarE;2differentially expressed genes and 5 differentially expressed proteins of PO.5.There were 104 significant differentially expressed genes among the three comparison groups of S-VS-Rh,S-VS-Rb and Rh-VS-Rb,which mainly focused on serine/threonine-protein kinase,DNA excision repair protein and transmembrane protease serine,protein-specific proteases,centriolar protein,myosin light chain,muscarinic acetylcholine receptors.6.For Pathway enrichment analysis of differentially expressed proteins,only metabolic pathway was obtained between the four comparison groups.This pathway includ metabolism of xenobiotics by cytochrome P450 and drug metabolism by cytochrome P450.The[2.5.1.18]pathway is most common in the metabolism of drugs and xenobiotics in CYP,and the up-regulated proteins in this pathway are closely related to the resistance to flubenzamide.