Study On The Location And Annotation Of MHC Gene And Vaginal Microecology In Alxa Bactrin Camel

The vaginal myiasis is currently known to be the most harmful parasitic disease to the Alxa Bactrin Camel.Epidemiological investigation showed that Alxa Bactrin Camel in the same community showed different susceptibility to myiasis.Around this phenomenon,this study conducted a related study on the location annotation of the MHC gene of Alxa Bactrin Camel and its vaginal microecology.Firstly,the location and annotation of MHC genes related to susceptibility and resistance of vaginal myiasis in Alxa Bactrin Camel were completed by comparative genomics.Then,high-throughput sequencing technology was used to analyze the vaginal micro-ecological flora,an important component of vaginal mucosal immunity in alashan bactrian camel,and the differences in the structure and diversity of vaginal microflora of illness camel and normal camel were compared.The ACQUITY UPLC H-Class Ultra Performance Liquid Chromatograph was then used to analyze the vaginal secretions of non-susceptible camels that are susceptible to camel and vaginal mycosis.Finally,three compounds matched in the metabolomics analysis were selected to study the tropism of female Wohlfahrtia magnifica on host pheromone volatile compounds.The results showed that 24 MHC genes in the genome of Alxa Bactrin Camel were found and annotated.However,in the affected group,bactrian camel's vagina pH was significantly decreased due to the significant increase of acid bacteria in the vaginal flora,and the level and genus in the sample group Levels identified 4 and 51 differentially significant taxon units,respectively.The changes in these flora may be related to immune regulation in the entire pathological cycle of vaginal myiasis in Alxa Bactrin Camel,and it is beneficial and positive.