Study On Inoculation-enhanced Composting Process And Conversion Of Carbon And Nitrogen Containing Matter

The purpose of this study was to screen and identify the functional microorganisms in composting and apply them to improve composting performance.Firstly,fungi and thermophilic bacteria were screened and identified,and a compost microbial bank was preliminarily established.Secondly,the functional characteristics of screened microorganisms were preliminarily understood by hot resistance test and enzyme activity analysis.Finally,the microbial compound microbial agent composed of functional strains was applied to enhance the degradation of lignocellulose and the humification process.Seventeen thermophilic bacteria and eight fungi were screened from dairy manure and sugarcane leaves compost samples.The laccase activity,filter paper enzyme activity,carboxymethyl cellulase activity and surfactant production performance of these microorganisms were investigated.Three strains of thermophilic bacteria were selected as follows:Ureibacillus.suwonensis(TB42),Geobacillus,thermodenitrificans(TB62)and Bacillus,licheniformis(TA65)to prepare microbial inoculant(TCMC),which was inoculated into dairy manure and sugarcane leaves composting in two-steps to study the effect of inoculation on physico-chemical parameters and nitrogen transformation as well as the evolution of dissolved organic matter.In addition,numerous studies have documented that B.licheniformis,A.nidulans and A.oryzae can secrete LPMOs.Thus,a bacterium(B.licheniformis TA65)and two fungi(Aspergillus,nidulans GXU-1 and Aspergillus,oryzae GXU-11)were selected to prepare microbial inoculant(CTMC)and inoculated into dairy manure and sugarcane leaves composting to investigate the influence of CTMC agent on composting humification process,lignocellulose degradation and microbial community structure succession.Composting is a typical microbial ecosystem,and microorganisms play an important role during composting process.In this work,microorganisms were screened from compost samples and applied to enhance composting process.The effects of inoculation on the succession of microbial community structure and the transformation of carbon and nitrogen during composting were further studied,which has certain significance for improving composting technology,understanding carbon and nitrogen conversion process as well as studying the succession of microorganisms during composting.