| In this study,nine rootstock pumpkin germplasm resources preserved by our research group were used as materials,and thin-skinned melon"Guangmi 1"and Thick-skinned Melon"Lucky 11”were used as scions respectively.The survival rate of grafted seedlings,resistance to Fusarium wilt,plant growth,yield and quality after planting in the field were studied.The grafting compatibility and symbiotic compatibility of nine rootstocks with melon were systematically studied.Sex provides experimental basis and theoretical reference for the breeding of special Rootstock Varieties for disease resistance of melon.The main results are as follows:The survival rates of grafting were calculated at 10,15 and 20 days after grafting.Except for NC02 and N03T,88.89%and less than 90.0%of the grafting survival rates of thin-skinned melon and N01 and thick-skinned melon,the survival rates of other numbered and Thick-skinned Melon and thin-skinned melon were higher than 90.0%,indicating that the grafting compatibility of 6 rootstock pumpkins with thin-skinned melon and Thick-skinned Melon was higher than 90.0%.It can be used as an alternative germplasm resource for selection of thin-skinned and thick-skinned muskmelon rootstocks.1.Resistance to Fusarium wilt of melon was identified by root injury method in artificial indoor seedling stage.The incidence of grafted seedlings was observed continuously for 21 days.The results showed that the incidence of self-rooted seedlings of two kinds of melon was above 80%,which were all susceptible.The grafted plants of Rootstock-scion combination showed high resistance,whether Thick-skinned Melon or thin-skinned melon.According to Koch's rule,a large number of pathogenic bacteria appeared from the rootstock,but few pathogenic bacteria were isolated from the stem of grafted seedlings with pumpkin as rootstock,which indicated that grafting melon with rootstock could effectively control melon wilt.2.The cultivation experiments of thin-skinned melon and Thick-skinned Melon Rootstock-scion combinations were conducted to study the effects of 9 rootstock pumpkin germplasm resources on the growth and development of melon grafted seedlings in the field.The physiological indexes such as plant height,stem diameter,leaf area,number of leaves,flowering period,chlorophyll,sucrose and starch content in leaves of grafted seedlings were measured.The comprehensive evaluation of membership function showed that 9 rootstock pumpkins were used as rootstocks.The growth vigor of Thick-skinned Melon and thin-skinned melon grafted on melon was better than that of their respective self-rooted seedlings.The Rootstock-scion combinations with NZ02,NC01?NC02,N03T and NC09 as rootstocks had better comprehensive performance in growth and development than other Rootstock-scion combinations.3.The results of yield and fruit quality of Rootstock-scion combinations showed that 9 rootstock pumpkins had no significant effect on the taste of Thick-skinned Melon and thin-skinned melon,and there was no significant difference in the taste of grafted seedlings compared with self-rooted seedlings;in mineral nutrition,different Rootstock-scion combinations showed different mineral element contents,and magnesium content in the fruit of thin-skinned melon and Thick-skinned Melon in each treatment.The nutrient quality of fruit(soluble solids,soluble protein,VC,free amino acid,soluble sugar,titratable acid,sugar-acid ratio)was evaluated by membership function.The results showed that the grafted plants with NC02,NC09 and N03T as rootstocks performed better in nutrient quality of fruit.Based on the results of the above four parts,NC09 could be used as excellent germplasm resources for grafting cultivation of thin-skinned melon in 9 rootstock pumpkins,NC02,NC09 and N03T could be used as excellent germplasm resources for grafting cultivation of thick-skinned melon. |
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