Protective Effect Of Taurine On Renal Injury Induced By AFB1 In Rats

Aflatoxin B1(AFB1)is a kind of mycotoxin with the strongest toxicity.AFB1 poisoning occurs after the intake of livestock and poultry,will reduce the body immunity,damage liver,spleen,kidney and other detoxification organ structure and function.The kidney is one of the detoxification organs of AFB1,and the metabolites of AFB1 are mainly excreted from the body by the kidney,and the residue in the kidney is very high,which can cause irreversible damage to the kidney.Taurine has a strong antioxidant ability,and has a good effect on reducing cell damage and preventing apoptosis.A large number of studies have also shown that taurine can prevent and protect kidney injury and nephrotoxicity caused by multiple chemical substances and heavy metal poisoning,but the protective effect and mechanism of taurine on kidney injury caused by AFB1 are still unclear.In this study,the rat model of AFB1 poisoning was prepared by gavage,and different doses of taurine were added to the drinking water to explore the protective effect and mechanism of taurine on AFB1 induced kidney toxic injury in rats,so as to provide theoretical basis for protecting the kidney injury caused by AFB1 by taurine in livestock and poultry breeding.Selected 77 healthy SD rats,and randomly divided into 7 groups,free to eat on the basis of given different treatment in each group: group C(gavage saline water,drinking water),group T(gavage saline water,drinked water 2% taurine),DMSO group(gavage DMSO,drinking tap water),group M(gavage AFB1,drinking water),M+T1 group(gavage AFB1,drinking water 1% taurine),M+T2 group(gavage AFB1,drinking water 2% taurine)and M+T3 group(gavage AFB1,drinking water 4% taurine).Mental state and body weight of rats were observed during the experiment;Microplate method and enzyme-linked immunoassay were used to detect the indicators of renal function in serum and urine and the indicators of oxidative stress in renal tissue.Test after maked tissue section HE dyeing observation kidney tissue morphology,colorimetric method and ELISA method determination of related parameters of renal function in serum and urine and kidney tissues of related parameters of oxidation ability,TUNEL method to detect renal cell apoptosis rate,real-time fluorescent quantitative PCR method to detect renal oxidative stress related factors and apoptosis pathways in related factor m RNA expression level.The test results showed that:(1)Taurine can inhibited the weight loss and renal index increase caused by AFB1 in rats,and improved the pathological changes such as renal cell vesicular degeneration and nuclear lysis.(2)Taurine inhibited the increase of blood BUN,CRE,Cys-C and UA,urine protein andAKP levels induced by AFB1.(3)Taurine inhibited the content/activity of CAT,GSH-Px,SOD,T-AOC,SDH and GSH,as well as the m RNA expression levels of SOD,GSH-Px,NQO1,?-GCS,HO-1 and GCLC in rat renal tissues induced by AFB1.MDA content,AKP activity and Nrf2 m RNA expression were inhibited.(4)Taurine inhibited the apoptosis rate of rat renal cells caused by AFB1 and the increase of the m RNA expression levels of Bax,Bak-1,Casp9,Casp3,Cyt-c and Apaf-1 in renal tissues,and prevented the decrease of the m RNA expression levels of Bcl-2 and Bcl-xl.The present results demonstrated that taurine can improve the renal function of rats with chronic AFB1 poisoning,which may be related to the activation of the Keap1-Nrf2/ARE signaling pathway to improve the antioxidant capacity of the kidney and inhibit mitochondria-mediated apoptosis.