Effects On The Intestinal Microbial Communities With DPV Infection In Duck

Duck plague virus(DPV),also known as duck enteritis virus(DEV),can cause an acute septic hyperemic infection(ie,duck viral enteritis)in ducks,geese,and geese.DEV)is one of the important pathogens that seriously threaten the development of the duck industry.At present,domestic and foreign scholars have carried out in-depth research on DPV,but mainly focused on the morphological structure,infection process and molecular detection of viruses,and rarely related to the study of the relationship between viral infection and intestinal flora,and intestinal flora.As an important part of inducing intestinal mucosal immune response in animals,it plays an important role in maintaining and ensuring animal health.To this end,this study used advanced Illumina Hiseq 2500 high-throughput sequencing technology to analyze the impact of DPV infection on the intestinal flora of ducks,in order to provide a scientific basis for the prevention and control of DPV infection.The 35-day-old healthy Cherry Valley ducks were randomly divided into two groups,the control group and the infection group.The infected group was intramuscularly injected with0.1 mL of DPV-GZ strain virus solution,and killed at 24 h,48h,72 h,96h and 120 h after inoculation.5 rats/(group ? time period),normal group intramuscularly sterilized saline,killed at 24 h after inoculation,collected samples of liver,spleen,brain and intestine contents,extracted viral DNA,and quantified by DPV-NP gene PCR method for detecting virus content;At the same time,the contents of the experimental duck intestine were collected and Sended to Guangzhou GENE DENOVO for high-throughput sequencing of 16 S rDNA V3~V4 hypervariable region.The bioinformatics software was used for analysis.The main results obtained are as follows:1.Changes in bacterial community structure of ducks infected with DPV:DEV was detected in duck liver and spleen 24 hours after DPV infection,and all tissues of ducks could be detected by 48 h.Alpha index analysis showed that with the increase of DPV infection time,the virus content increased,and the intestinal flora of ducks was abundant.The degree anddiversity index(Chao1,ACE,Shannon,and Simpson)showed a rising and then decreasing trend;According to the significant difference analysis of the flora structure,the intestinal flora of the infected group was not significantly different from the normal group at 24 h after infection,and was significantly different at 48 h and 72 h,and was different at 96 h and 120 h after infection.Significant.This suggests that DPV infection can lead to structural changes in the gut flora of ducks.2.Bacterial abundance changes in duck gut infected with DPV:Analysis by bacterial species showed that DPV infection had a certain effect on the relative abundance of colonies in duck intestines.Among them: at the level of the phylum,Firmicutes first increased and then decreased,Fusobacteria and Bacteroidetes increased,and Proteobacteria decreased;at the level of the class,Clostridia increased first and then decreased,and Bacteroidia and Fusobacteriia increased,and Gammaproteobacteria,Erysipelotrichia and Bacilli decreased;at the level of the order,Clostridiales and Selenomonadales first increased and then decreased,Bacteroidales and Fusobacteriales increased,Enterobacteriales,Selenomonadales and Lactobacillales were decreased;at the level of the family,Bacteroidaceae,Fusobacteriaceae and Ruminococcaceae increased,Peptostreptococcaceae and Rikenellaceae first increased and then decreased,Enterobacteriaceae and Erysipelotrichaceae are decreased;at the level of the genus,Fusobacterium and Bacteroides increased,Escherichia-Shigella and Candidatus_Arthromitus are decreased,Alistipes,Megamonas,Turicibacter and Faecalibacterium first increased and then decreased;at the level of the Species,Clostridium_perfringens and Fusobacterium_mortiferum increased.3.DPV-infected duck intestinal major difference bacterial community:According to LEFse analysis,the main differential bacterial communities are:Proteobacteria,Gammaproteobacteria,Enterobacteriales,Enterobacteriaceae,Escherichia-Shigella,Bacilli,Lactobacillales;Fusobacteria,Clostridia,Fusobacteriales,Fusobacteriaceae,Fusobacterium,Campylobacterale-s,Campylobacteraceae,Arcobacter,Clostridium_perfringens.In summary,DPV infection can lead to changes in the structure of the intestinal flora of the duck,and the infection time is prolonged,and the difference in the intestinal flora of the duck is more significant.These findings suggest that the development and progression of theDP course can be prevented and controlled by maintaining and stabilizing the structure of the duck gut flora.