Functional Study Of The Transcription Factor TaNAC025 In The Interaction Between Wheat And Stripe Rust

Transcription factors play a crucial role in plant development,growth,and response to various biotic and abiotic stress factors.Wheat is a major food crop in China and in the world.Wheat stripe rust caused by stripe rust wheat specialization is an important disease threatening the safety of wheat production in China.To clarify the biological functions of transcription factors in the process of resistance and stress resistance of wheat,and provide disease resistance genes for stripe rust breeding.This study used bioinformatics methods to search for genomic-level NAC transcription factors and using the cDNA library of the near-isogenic line Taichung29*6/Yr10 of wheat resistance to stripe rust gene Yr10 induced by stripe rust race CY32 constructed in the early stage of the laboratory,Screened a wheat transcription factor gene TaNAC025.The expression profile analysis of TaNAC025,the interference of TaNAC025 expression by BSMV-VIGS technology system and the resistance to stripe rust of gene gun-mediated over-expressed plants were carried out to verify the disease resistance function.The main findings include:1.Based on the genomic data of the common wheat "Chinese Spring"(Ensemble release-41),the hidden whole Markov model was used to search for NAC-like transcription factors in wheat genomes,and 448 NAC genes were obtained.Among them,185 TaNACs(41.43%)are newly discovered NAC members.Clarify Chromosome location,gene structure,conserved protein-based motifs,and gene replication analysis of each gene.Among them,155 TaNACs were located in the A genome,141 TaNACs were located in the B genome,and 140 TaNACs were located in the D genome.The MEET program was used to search for Motif in the 448 TaNACs,and 10 conserved motifs were obtained.The evolutionary characteristics of the NAC gene were systematically studied.By analyzing the existing RNA-seq data,the expression of NAC in the interaction between wheat and stripe rust was studied.34 genes were selected and their expression in the infection process of stripe rust was verified by qRT-PCR.2.TaNAC025 belongs to the NAP subgroup of the NAC transcription factor family.TaNAC025 is mainly expressed in the leaves of wheat senescence.The expression level of TaNAC025 was significantly up-reguLated after wheat inocuLation with Puccinia striiformis or spraying of exogenous phytohormone ABA.After using the BSMV-VIGS technology system to interfere with the expression of TaNAC025,after inocuLation with stripe rust,the spore heap of the leaves interfering with TaNAC025-expressing plants was larger and the number of spores per unit area was larger.Therefore,TaNAC025 is reguLating the resistance of wheat to stripe rust.At the same time,TaNAC025 was found to delay the dark-induced leaf senescence and reguLate the senescence process of wheat.Eight T3 generation homozygous family materials overexpressing TaNAC025 were obtained by gene gun method.After inocuLation with the affinity strain,the stripe rust of TaNAC025 overexpressing plants was less severe and the number of spore heaps was less,that is,the resistance of overexpressing plants to stripe rust was significantly enhanced.Further analysis of the relevant basic defense genes indicated that the background expression levels of PR1 and PR5 in overexpressing plants were significantly increased,that is,TaNAC025 may increase the resistance of wheat to pathogen infection through SA signaling pathway.3.The qRT-PCR method was used to identify the expression levels of 12 similarity genes to TaNAC025 in wheat and stripe rust.Induction of 11 genes by infection by stripe rust.Using BSMV-VIGS technical system analysis,it is found that it plays a positive role in the interaction between wheat and stripe rust.