Whole-genome Mining Of GATA Transcription Factor From Tomato And Screening Of Resistance-related Genes

A GATA transcription factor exists widely in eukaryotes,and it participates in some important biological processes,for example,plays an important role in plant responses to light,chlorophyll synthesis,cytokinin responses,resisting biotic and abiotic stress,and carbon and nitrogen metabolism,which are biological processes that are closely related to crop production.GATA transcription factors have been reported in other creatures,but there is very little analysis of GATA transcription factors in tomato.Therefore,this study aims to fully mine and identify GATA transcription factors in tomato,and through bioinformatics analysis,the relevant characteristics of family genes were identified,and the family members related to stress-resistance of tomato were screened and identified,and some genes that had significant resistance to stress were functionally verified.The aim is to lay a foundation for the research and utilization of tomato resistance stress genes.The concrete results are as follows:(1)By means of bioinformatics method,this study identified 30 members of the GATA transcription factor family in tomato.The family members could be divided into 4 subgroups.Most of the GATA transcription factors in tomato were conserved.Chromosome location analysis showed that tomato GATA transcription factor was distributed on 12 chromosomes of tomato,but the number was different.(2)The tissue-specific expression patterns of 14 GATA members of subfamily I in tomato were analyzed.The results showed that the expression of these genes in various tissues of tomato was very different.The relative expression of the same gene in different tissues was very different,Meanwhile,there are differences in the expression of different genes in the same tissue.For example,the SlGATA11 gene is almost only expressed in the flower.(3)In this study,all the gene members of the subfamily I were selected to analyze the expression pattern of GATA transcription factors in tomato under abiotic stress.Under salt treatment,the change of expression of most of the genes was not very obvious;Under cold conditions most of the gene expression changes are significant.The up-regulated expression of SlGATA11 and SlGATA26 is the most significant;Under drought conditions,the expression of 6 genes(SlGATA7,SlGATA2,SlGATA13,SlGATA17,SlGATA22,SlGATA11)was up-regulated.The expression of SlGATA7,SlGATA17,SlGATA22,SlGATA13 and SlGATA11 first increased and then decreased.(4)This study analyzed the gene SlGATA17 with significant change and obvious change trend in drought stress response analysis,and using VIGS technology to verify its function.It was found that the down regulated expression of SlGATA17 gene reduced the drought resistance of tomato plants.