Bioinformatics Analysis Of Tomato G2-like Transcription Factor Family And Identification Of Resistance-related Genes

As a kind of worldwide crop,tomato occupies an extremely important position in people's daily diet.The way of eating tomato is very varied,and its edible value is very high,which determines its important status.But in the process of its cultivation,it will suffer from the harsh living conditions caused by environmental changes,which has a huge impact on tomato production.Many studies have shown that some transcription factors are related to the growth and development of plants.Transcription factor is a kind of protein that can bind to a promoter to regulate the expression of certain specific genes,thereby affecting plant growth and response to unfavorable growth conditions.For tomato,improving the tomato's resistance to unfavorable growth conditions and trying to minimize damage to tomato growth caused by adverse conditions,which will be of great significance for tomato breeding.This paper analyzed the expression of some genes of the tomato G2-like transcription factor family under three stresses to determine whether these genes respond to stresses,and some genes that may be related to the resistance of tomato were obtained.Then we carried out the preliminary functional analysis of SlGlk29 to verify its role in tomato resistance.(1)54 G2-like transcription factor family members were identified in the whole genome of tomato.According to the G2-like family classification in maize and the motif analysis of tomato G2-like family members,54 members were divided into six subfamilies.(2)In the tomato G2-like transcription factor family,15 members of representative I-V subgroups were selected to analyze their expression levels in various tissues(roots,stems,leaves,flowers,green fruits,red fruits,and sepals).All genes showed different expressions in the same tissue and in different tissues.The expression of G2-like genes was the most uniform in flowers and the lowest in stems.(3)After cold treatment,the expressions of SlGlk1,SlGlk7,SlGlk34,and SlGlk37 were up-regulated,the expressions of SlGlk23,SlGlk21,and SlGlk35 were down-regulated and the expressions of SlGlk29 and SlGlk42 increased first and then decreased;after drought treatment,the expressions of SlGlk7,SlGlk15,and SlGlk41 were up-regulated,the expression of SlGlk25,SlGlk40,SlGlk21,SlGlk35,and SlGlk42 genes was down-regulated and the expressions of SlGlk33,SlGlk29,and SlGlk37 genes increased first and then decreased;after salt treatment,the expressions of S1Glk7,SlGlk15,SlGlk33,SlGlk36,SlGlk34,and SlGlk35 were up-regulated,the expressions of SlGlk21 and SlGlk29 were down-regulated and the expression of SlGlk41 increased first and then decreased.(4)The pTRV2-SlGlk29 vector was successfully constructed and the silencing plants were obtained for the SlGlk29 gene with significant response patterns in drought and cold stress.The results of analysis of plant phenotype and related physiological and biochemical indicators after stress stress indicated that the down-regulation of SlGlk29 gene reduced the resistance of tomato plants to drought and low temperature.