| Bactrocera dorsalis(Hendel)is a serious boring pest.Using Sterile Insect Technique(SIT)to control the B.dorsalis is a safe and effective means of scientific,this technology has been successfully applied in a variety of pest control in the Tetriphitidae.However,the sterile male released to the field may be damaged in the process of sterility treatment,making it inferior to the wild male in survival rate,mating competitiveness and other aspects.In this study,Illumina HiSeq high-throughput sequencing technology was used to analyze the changes in the diversity and abundance of intestinal flora of male sterile flies of B.dorsalis after irradiation.In addition,the effect of intestinal microflora on pupal weight,emergence rate,survival rate under pressure,competitive mating ability and life span of male B.dorsalis was preliminarily verified.Male B.dorsalis were fed with the isolated strains in the larva and adult feeds to assess their health fitness to male B.dorsalis.The research results are as follows:1.Illumina HiSeq sequencing was used to analyze the diversity changes of intestinal flora.The results showed that all OTUs and specific OTUs in the irradiated intestinal flora were less than those in the control group,and the shannon index,simpson index and chaol index of intestinal samples of sterile males in the irradiated group were lower than those in the control group,which reduced the diversity and richness of intestinal bacteria of sterile males.Proteobacteria and Firmicutes respectively by 60.376% and 24.330% as advantage of infertile male intestinal bacteria and dominant Phylum,Enterobacteriaceae and Enterococcus at 60.376% and 15.694% respectively as the dominant Family bacterium,Enterobacter and Vagococcus is at 33.840% and 12.530%,respectively,as the dominant Genus.In addition,Moellerella wisconse,Morganella morganii,and Cosenzaea myxofaciens were significant difference Species.The the abundance of Moellerella wisconse increased significantly after irradiation,while Morganella morganii and Cosenzaea myxofaciens abundance of significantly reduced after irradiation.2.The results showed that the male B.dorsalis in the antibiotic treatment group were significantly lower than those in the control group in terms of pupal weight(P=0.001),pressure-resistant survival rate(P=0.011)and competitive mating ability(P=0.001).In addition,the head width(P=0.002),thorax length(P=0.003),abdomen length(P=0.003)and wing length(P=0.012)of sterile males in the treatment group were significantly smaller than those in the control group,and there was no significant difference in eclosion rate between the treatment group and the control group(P=0.397).In addition,the treatment group male B.dorsalis life time was significantly lower than the control group.3.A total of 17 strains were successfully isolated by combining traditional microbial isolation and culture methods with l6 SrDNA gene sequence identification.E.asburiae,E.ludwigii,E.cloacae,E.bugandensis,E.hormaechei,Enterobacter sp.,E.cancerogenus,E.aerogenes,Escherichia hermannii,Citrobacter freundii,Pantoea sp.,Morganella morganii,Moellerella wisconsensis,Klebsiella sp.Providencia sp.,Enterococcus faecalis,Lactococcus lactis.Enterobacter was the dominant bacterium in the intestinal flora of B.dorsalis.4.The isolated Enterobacter(8 strains),Morganella(1 strain)and Moellerella(1 strain)were added to the larvae feed and the adult feed to evaluate the effects on the health fitness of the irradiated B.dorsalis males.The male B.dorsalis pupa weight and adult size,survival and competition ability and so on have significant enhancement of the mating,and eclosion rate and the ability to fly and no obvious enhancement.Instead,Morganella morganii and Moellerella wisconsensis two strains of bacteria after irradiation of the B.dorsalis male has significant negative effects. |
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