Molucular Colning And Expression Anaylysis Genes Of TMS?10 And RPL36 In Liaoning Cashmere Goat

Liaoning cashmere goat is famous for its high cashmere yield and long fibre,and is one of the most important genetic resources.But low fineness of cashmere affected its economic performance.Based on the measure of cashmere diameter,the coding sequence of DS of TMS?10 and RPL36 were cloned and analyzed with the bioinformatics software,and the expression of them in different tissues and in skin,hair follicle and dermal papilla cells from different fineness group were also detected.this research would lay foundation for further genetic mechanism research in fineness trait for cashmere goat.The result were as follows.1.The diameter of cashmere was 17.70 �1.59 ?m,which complied with breed standard of Liaoning cashmere goat.2.The coding sequence of TMS?10 was 129 bp,Sequence analysis found that the homology between it and human,pig,bovine,predicted goat sequence reached 86.8%,96%,99%,100%,respectively.The sequence coded 42 amino acid residues.The molecular weight of TMS?10 was 4.67 kDa,and the theoretical pI was 6.5.TMS?10 was a hydrophobic protein without signal peptide and transmembrane area,and mainly composed of alpha helical and irregular curl.TMS?10 was expressed in heart,liver,spleen,lung,kidney and skin,and the highest level was in lung.The expression level was lower in skin,hair follicle from higher fineness group than from the lower fineness group,but the expression level in dermal papilla cells represented the results as opposed to it in skin and hair follicle from different fineness group.3.The coding sequence of RPL36 was 318 bp,Sequence analysis found that the homology between it and human,pig,bovine,predicted goat sequence reached 91%,92%,99%,100%.The sequence coded 105 amino acid residues.The molecular weight of RPL36 was 12.22 kDa,and the theoretical pI was 11.59.RPL36 was a hydrophobic protein without signal peptide and transmembrane area,and mainly composed of alpha helical and irregular curl.RPL36 was expressed in heart,liver,spleen,lung,kidney and skin,and the highest level was in lung and skin.The expression level was lower in skin,hair follicle from higher fineness group than from the lower fineness group,but the expression level in dermal papilla cells represented the results as opposed to it in skin and hair follicle from different fineness group.