Study On The Expression Of MP1 In Liaoning Cashmere Goat Skin

Liaoning cashmere goat is the precious genetic resources of China which is banned outflow. Cashmere production ranks first in the country and has great economic value. Therefore, it will be more practical significant to study the function of gene related to the pashm.In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed MP1(MEK Partner 1). Firstly,we have a research on the cDNA and amino acid sequences by bioinformatics analysis, and establish evolutionary tree and so on. Otherwise, RT–PCR is used to analyze the expression of MP1 gene in skin,hert,liver,spleen,lung and kidney. Real-time RT-PCR is used to detect the relative quantitative expression of MP1 gene in the primary and secondary hair follicles during the anagen and catagen two growth periods. Finally, The expresion site of MP1 gene is analyzed by In situ hybirdization.In addition,object to study whether there are some relationship between MP1 gene and cashmere fineness, we make use of a specific lentiviral RNAi expression vector targeting Noggin gene and verify its regulating effect on MP1.Then we can provide an important theoretical base to improve cashmere having excellent economic properties.The results are as follow:(1)The bioinformatics analysis show that MP1 could encode the protein which including 124 amino acids.It is a kind of hydrophily protein. Secondary structure showed it had Alpha helix(60.33%),Extended strand(33.87%),Randon coil(40.32%).Function site analysis showed that MP1 had no transmembrane domain and signal peptide. The protein homology analysis show that MP1 included a conservative MAPKKl-Int domain is of high conservative in different species,and has 3 Phosphorylation sites.Semi-quantitative RT-PCR showed that MP1 is expressed in heart and skin,but no expression in liver, spleen, lung, kidney tissues. The expression of MP1 by qPCR in the second follicles was 1.65 times higher of primary hair follicles during anagen(P<0.01); the expression of MP1 in the second hair follicles was 3.25 times of the primary follicles during catagen(0.01<P<0.05). Moreover, MP1 was obviously expressed in the Inner Root Sheath(IRS) but no expression in the hair shaft, Outer Root Sheath(ORS), or Follicular Papilla(FP).(2)We successfully construct the lentiviral vector containing Noggin gene, the relative expression of MP1 is increased by qPCR.We can draw the following conclusions by the above results,(1) MP1 may have a effect on skin cell growth,involved in hair follicle re-epithelization,and have the function of making the hair thin.(2)MP1 gene may be located in downward trend of Noggin, by means of the relationship with Noggin gene,enforce the hair follicles of the steady state and development.