Font Size: a A A

Genetic Diversity And Phylogenetic Relationship Analysis Of Plum Germplasm Resources

Posted on:2019-12-04Degree:MasterType:Thesis
Country:ChinaCandidate:J WangFull Text:PDF
GTID:2393330596955986Subject:Garden Plants and Ornamental Horticulture
Abstract/Summary:PDF Full Text Request
In order to assist the management of plum germplasm resources and elucidate the interspecies phylogenetic relationships,two molecular markers such as ISSR and RAPD were employed to detect the genetic diversity in 155 accessions of plum germplasm resources.The accessions tested consisted of 6 plum species belonging to Prunus salicina,Prunus cerasifera,Prunus domestica,Prunus ussuriensis,Prunus armeniaca and natural/artifical hybrids between apricot and plum.1.A total number of 440 bands were generated using 25 ISSR primers for 155 accessions,with an average number of 17.6 per primer,among which 94.32% of the total bands was polymorphic and a total number of 226 bands using 16 RAPD primers for 155 accessions,with an average of 14.1,among which 94.25% was polymorphic.The Jaccard's coefficients of similarity between accessions tested ranged from 0.543 to 0.921 for ISSR,and from 0.557 to 0.898 for RAPD.The efficiencies of two markers for identication of plum germplasm were ISSR>RAPD.RAPD and ISSR were suitable for utilization in genetic diversity analysis and genetic relationship identification of plum germplasm resources uncomplicated,rapid and economical methods if much were identical for all reactions.2.ISSR genetic diversity analysis was performed on 155 pieces of plum material.When the similarity cofficient value was 0.71,the 155 accessions plum were divided into four groups.The main coordinate classification result was consistent with it.The PPB,H and I levels of individual germplasm resources in the ISSR test were 94.32%,0.266,and 0.401,respectively,which were generally higher than other populations,indicating that the genetic diversity at the individual level was higher than the population level;The level of genetic diversity of plum germplasm resources at the level of origin in Jilin Province(90.23%,0.258,0.391)was greater than those of Heilongjiang(80.91%,0.260,0.390)and in other region(85.23%,0.273,0.408).Germplasm resources,also could be seen at the level of cultivar group and individual,the phenotypic diversity index I was higher than the genotype-based diversity index H.3.RAPD genetic diversity analysis was performed on 155 pieces of plum material.When the similarity cofficient value was 0.69,the 155 accessions plum were divided into four groups.When the similarity cofficient value was 0.72,the first component can be further divided into 7 subgroups.The main coordinate classification result was consistent with it.The PPB,H and I levels of individual germplasm resources of RAPD tested were 94.25%,0.236,and 0.368,respectively,which were higher than the corresponding index of population,indicating that the genetic diversity at the individual level was higher than the population level;The genetic diversity of plum germplasm resources originating from the Jilin area(91.15 %,0.236,0.368)was greater than that of the Heilongjiang(84.07 %,0.232,0.359)and other regions(86.28 %,0.231,0.359).At the cultivar group and individual level,the phenotypic diversity index I was higher than the genotype-based diversity index H.4.The morphological indicators of the 151 germplasm resources of the study were analyzed.For Q-type cluster analysis of 45 morphological traits,151 materials were divided into two groups with a value of 20,the first group was Ziye,and the other was Yipindanfeng,92-1-2,92-1-3,92-1-1,92-1-9,92-1-10;other varieties were clustered together.With 16 as the threshold,151 materials were divided into seven groups.R-type clustering results,with 3 as the domain value,were divided into three categories.Principal component analysis obtains the eigenvalues,contribution rates,and cumulative contribution rates of each principal component.The first 15 principal components reflected the original 67.326% of the data information.The combination of morphological markers and molecular markers facilitates the identification and analysis of the genetic diversity and genetic relationship of plum germplasm resources.
Keywords/Search Tags:plum, molecular markers, morphological classification, genetic diversity, phylogenetic relationship
PDF Full Text Request
Related items