Phylogenetic Relationship And Classification Analysis Of The Native Persimmon (Diospyros Kaki Thunb.) In Zhejiang Province Based On SSR Markers

Persimmon (Diospyros kaki Thunb.), as one kind of economic forest tree species representing endemicspecies of Zhejiang province, is cultivated and distribute63counties in ten district because of its adaptation.However, the mojority of existent native persimmon germplasm resources are seeding posterity notcross-breeding, and agronomic trait is grafted year by year, that makes the phenomenon of synonyms orhomonyms serious and relationshiop unclear. Exchange among different areas, lack of uniformed namingsystem, high bud spots in occurence is another reason for that. Therefore, it is necessary to carry out thereseach on phylogenetic relationship and classification, which is of impotant guiding significance forextension of fine varieties and innovation of extant gerplasm.In this study, a total of214materials were studied, and53pair SSR molecular markers,developed fromthe genus of Diospyros, were screened to evaluate phylogenetic relationship of landrace and species andclassification of similar varieties. The germplasm resources of Jinzaoshi persimmon in Zhejiang Provincewere also investigated and researched. A total of37Jinzaoshi persimmon were collected. Genetic variationsamong individual were probed by SSR molecular marker，together with phenotypic traits and fruit nutrientscontents. the main results were as follows:1.21pairs of polymorphic primers were screened from53ones.21primers amplified378polymorphic bands in214materials, with an average polymorphic rate of98.79%,18alleles per one primer.In the Dendrogram from the UPGMA cluster analysis, the material can be divided into two groups, varietiesand wild persimmon and three species (D. glaucifolia, D.lotus, D.oleifera) at a0.37threshold of similaritycoefficient.The varieties and wild persimmon were further subdivided into three subgroups(A, B, C),subgroups A included Group1, Group2, Group3. The relationship, reflected from two-dimensional andthree-dimensional principal coordinates analysis, corresponded to the result of UPGMA clustering analysis.2. The germplasm resources of persimmon in Zhejiang province had a rich genetic diversity.196persimmon varieties can be classified as174, of which65high phenotypic similarity persimmon varietiesclassified as50by SSR markers. 3. Twenty-one primers were used to amplify381SSR local in40materials, with an averagepolymorphism rate of92.89%. The cluster analysis conducted by UPGMA showed that the tested materialscould be distinguished and were divided into three major groups at the genetic similarity coefficient of0.74,which was similar to the result of PCoA analysis. The results showed Jinzaoshi persimmon possess a richdiversity on morphology. The variable coefficient about phenotypic character changed from7.23%to23.54%. The variable coefficient of sepals long and persimmon calyx long were the largest. Fruit shapeindex and leaf index had the least variation. Fruit nutrients contents ranged from9.58%(soluble solid)to66.21%(tannin content), the average coefficient of variation was37.89%. The variation degree of fruitnutrients contents higher than that of phenotypic traits.