Analysis Of Genetic Diversity Of Xanthoceras Sorbifolium In Eastern Inner Mongolia

Xanthoceras sorbifolium is an important ecological and energy tree species in northerm China.The eastern part of Inner Mongolia is the earliest and largest area of artificial cultivation,and there are also natural forests distributed over a thousand acres.It is of great significance for the analysis of genetic structure among different populations.The experimental materials were selected from eight different areas in the main cultivated areas of easterm Inner Mongolia.The genetic diversity and genetic relationship of the eight populations were studied from the perspective of morphology and molecular markers.The results are as follows:(1)Compared with the 14 indicators of 8 populations,annual seedling traits and biomass,the seeds,annual seedling traits and biomass indicators of C-Z-T population were higher than C-Z-R1972 population;X-Q-R1976 has the highest indices of sporniodenn thickness,seed vigor,annual seedling height,ground diameter,main root length and biomass.The seedlings of it has developed roots,strong drought tolerance and the best growth potential.The morphological feature clustering can be two types.The X-Q-R1976 population is a self-contained group,and the other seven populations are clustered into one category,that is,the X-Q-R1976 population and the other seven populations have different morphological characteristics.The morphological characteristics of the populations are similar.The environment has a certain influence on the morphological characteristics.(2)Tiangen new plant genomic DNA extraction kit improves the test efficiency and safety,the extracted DNA sample is pure and free of impurities.No phenol/chloroform extraction is needed in the process of the trail,the operation and the steps are simple.DNA samples that can be directly used in subsequent tests can be obtained in a short time.The DNA samples obtained by the improved CTAB method has a higher concentration,which may cause dragging and tying,so needs to be diluted before being used in subsequent experiments.And improved CTAB method requires phcnol/chlorofonn extraction,which takes a long time with complicated steps.(3)Thirteen pairs of SSR primers with high polymorphism,clear band and high stability were screened and 231 Xanthoceras sorbifolium Bunge materials were amplified.A total of 228 alleles were detected,the percentage of polymorphic loci(P)in each population was 100%.(4)Use GenALEx6.501 analyzed the genetic diversity,the results showed that there were differences in genetic diversity among populations.T-Z-R1977 and C-A-R2010 were higher,C-A-R2007 was lowest.(5)The SSR-PCR amplification product atlas of 231 individuals from 8 populations were analyzed by NTSYS.Classification at the similarity coefficient of 0.71 can be divided into two groups:C-Z-T and C-Z-R1972 were clustered into first group and the others were clustered into the second group;Classification at the similarity coefficient of 0.73 can be divided into three groups:C-Z-T and C-Z-R1972 were clustered into the first group;C-A-R2010,C-W-R1973 and X-Q-R1976 were clustered into the second group;C-A-R2007 and T-Z-R1977 were clustered into the third group.