Effects Of Salinity Changes On Enzyme Activity And Gene Expression In Scallop

Crassadoma gigantean belongs to the Mollusca,Lamellibranchia,Pterioida,Pectinidae,and Crassadoma genus,native to the Pacific coast of North America.The rocky scallop camp has a wide range of temperature and is suitable for wide-temperature species.The rock scallop has a large closed-shell muscle.The meat is delicious and has a unique flavor.It is very popular in the market and is in short supply.Moreover,because it grows fast It has strong adaptability to the environment.Therefore,rock scallop can be introduced as a good candidate for shellfish breeding.In 2012,in order to effectively alleviate the shortage of scallop breeding varieties,frequent disease,low yield and high mortality in China,rock scallops were introduced into China as an excellent variety.Rock scallop is a new variety introduced in China.In order to make up for the gap in the impact of China's domestic salinity on rock scallops,this experiment was carried out using the Crassadoma gigantea introduced from Canada.The experiment included salinity changes on the rock scallop SOD,Na+/K+-ATP and The determination of the effects of three enzyme activities of glutathione reductase and the transcriptome sequencing analysis were two parts.In the first part of the determination of the change of salinity on the three enzyme activities of rock scallops SOD,Na~+/K~+-ATP and glutathione reductase,the experiment was divided into salinity sudden change experiment and salinity gradual change experiment.For the gradients of 24,30,and 36,we obtained the following conclusions in the experiment of salinity sudden change:1)The activity of Na~+/K~+-ATPase at the salinity of 24 decreased first,then increased and then decreased.At the salinity of 30,the enzyme activity showed a gradual increase,and when the salinity was 36,the enzyme activity showed a small increase and then decreased.2)The activity of SOD at the salinity of 24 showed a trend of increasing first,then decreasing and rising.When the salinity was 30,the enzyme activity showed a steady upward trend.At the salinity of 36,the enzyme activity increased slightly and then decreased.The trend of rising and then falling.3)The activity of GR at the salinity of24 showed a trend of rising first and then decreasing again.When the salinity was 30,the enzyme activity showed a slow upward trend.When the salinity was 36,the enzyme activity increased first and then decreased.the trend of.In the salinity gradient experiment,we obtained the following conclusions:1)The enzyme activity of Na~+/K~+-ATPase showed a gradual decrease when the salinity was 24,and the enzyme activity did not fluctuate significantly when the salinity was 30.The salinity was 36.The enzyme activity showed a trend of decreasing first and then increasing slightly and then decreasing.2)When the salinity was 24,the enzyme activity showed a slow decline first and then a slight increase.When the salinity was 30,the enzyme activity did not fluctuate significantly.When the salinity was 36,the enzyme activity showed a slow decrease.3)When the salinity is 24,the enzyme activity shows a slight downward fluctuation and then slowly decreases.When the salinity is 30,the enzyme activity does not fluctuate significantly.When the salinity is 36,the enzyme activity first rises slightly and then slowly decreases.the trend of.Based on the comprehensive salinity change and gradual change experiments,we conclude that:1)In the comparison of three salinity gradients,the salinity of 30 is most suitable for rock scallop life.2)In the comparison of salinity gradients 24 and 36,the salinity of24 is more suitable for rock scallop life,that is,rock scallop is more resistant to low salt.In the second part of the second-generation transcriptome sequencing experiment,we obtained the following results:1)A total of 1089 differential genes were obtained from the sequencing results,349 genes were up-regulated,and 740 genes were down-regulated,of which 55 were successfully annotated into GO classification.2)Down-regulation of differentially expressed genes in normal salinity 30 to low salinity 24 GO clusters Mainly enriched in metabolism,single organism transport,cell membrane,adhesion,catalytic activity-related GO term,30 pairs in normal salinity Down-regulation of differentially expressed genes in GO clusters with high salinity of36 is also mainly enriched in appealing to several related GO terms,but more enrichment in metabolism,catalytic activity-related GO term,ie high salt and low salt water The environment inhibits a series of biological metabolic reactions of rock scallops and is more pronounced in high saline environments.3)The 20 pathways that were successfully annotated to the most significant enrichment on KEGG were analyzed and some related genes were found.For example,MyD88 gene was down-regulated in the low-salt group and the high-salt group.This gene is important for participating in the immune response.The gene,AdPLA gene also has a significant down-regulation,and an increase in the expression of AdPLA prevents fat breakdown.It is indicated that the high salt and low salt environment not only has obvious inhibitory effect on the immune function of rock scallop,but also is not conducive to the fat accumulation of scallop.In this experiment,the effects of different salinity gradients on the activity of SOD,GR,Na~+/K~+-ATP in rock scallop and the comparative analysis of differential genes were studied.It is hoped to provide a basis for the biochemical indicators and gene expression research of rock scallop,which will be introduced in the future.Provide guidance on production practices such as domestication,artificial nursery and adult farming.