| Cervus elaphus yarkandensis is a endemic subspecies of Cervus elaphus in xinjiang.It is mainly distributed in Tarim River Basin,Tarim Basin,Xinjiang.It has extremely high economic value and medicinal value.Piroplasms infection of the Cervus elaphus yarkandensis can cause clinical symptoms such as high fever,anemia,and jaundice,resulting in reduced production performance and even death,which seriously hinders its healthy and sustainable development.At present,domestic researchs on Cervus elaphus yarkandensis is mainly focused on breeding,breeding and habitat ecology,but there are few reports on Piriformosis.The purpose of this study is to understand the prevalence of Piriformosis of Cervus elaphus yarkandensis,and to provide basic information for the prevention and control of Cervus elaphus yarkandensis Piriformosis.In this study,blood samples were collected from nine large-scale Cervus elaphus yarkandensis farms in Bazhou,and tested by PCR method to grasp the prevalence and infection types of Piroplasms.1.In order to understand the infection of Piriformosis in Cervus elaphus yarkandensis,480 Cervus elaphus yarkandensis blood samples collected from 9 sampling locations in southern Xinjiang Production and Construction Corps were examined by PCR based on the common primers of Piroplasms.The results showed that out of 480 red deer blood samples,220 samples were positive for Piroplasms,with a total infection rate of 45.8%(220/480).There were significant differences in the infection status of piroplasms in Cervus elaphus yarkandensis at different sampling points(P <0.05).The highest infection rate of piroplasms in 34 groups was 93.8%(45/48),and the lowest was 10.5% in 30 groups(4/38).The results of the study showed that the infection of Piroplasms in Cervus elaphus yarkandensis is common,and the testing in clinical production should be strengthened.In this study,we learned about the infection of Piroplasms in Cervus elaphus yarkandensis in the Tarim region,which enriched the epidemiological data of Piriformis,and provided a reference for the prevention and control of Piroplasms.2.In order to further understand the species of Piroplasms infected with Cervus elaphus yarkandensis,PCR amplification was performed on 220 positive samples of Piroplasms,using Theileria species-specificprimers.As a result,only T.capreoli was detected,and the total infection rate was 45.8%(220/480).Then,the positive samples amplified by PCR are sequenced,and the sequencing results were searched for homologous sequences on GenBank and performed sequence alignment,it was found that all sequences are100% homologous with T.capreoli(MN463019),and identified as T.capreoli.The constructed phylogenetic tree showed that they are all located on the same branch as the T.capreoli.The results showed that the Piroplasms dominant species of the Cervus elaphus yarkandensis were T.capreoli.The results of the study provided basic information and data for the identification of Piroplasms in deer.3.In order to understand the actual effect of Diminazene Aceturate on the treatment of Cervus elaphus yarkandensis Theileriosis.In this study,the Theileria infection of 20 Cervus elaphus yarkandensis was selected as the study object and detected before and after the treatment by means of microscope observation and PCR detection.It was found that the positive rates of blood smear test and PCR test after medication were 0.0%(0/20)and 15.0%(3/20),respectively,which were lower than the positive rate of blood smear test before medication(35.0%,7/20)and the positive rate of PCR detection method(70.0%,14/20),indicating that Diminazene Aceturate had a good therapeutic effect on Cervus elaphus yarkandensis Theileriosis,which provide a basic reference for the prevention and control of Theileriosis in deer in China. |
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