| Tomatoes are native to the andes regions of Peru and Ecuador.Originally cultivated as an ornamental plant,the tomato spread to other parts of the world and was cultivated as a food crop in the mid-18 th century.Tomato has rich nutritional value and contains lycopene,which has a unique antioxidant capacity.Now tomato has become an indispensable vegetable in people's life.However,in the process of tomato growth,the emergence of adverse environment(drought,flood and soil salinization,etc.)will lead to a significant decrease in tomato yield and quality,which will seriously restrict the development of agricultural economy.The growth and development of plants cannot be separated from the regulation of auxin,which can promote the growth,differentiation and fruit development of cells,apical dominance and directional growth.The action of auxin depends on its concentration and distribution in plant tissues and cells.Tomato PIN protein family is a recognized auxin efflux carrier.As a transport protein,it can control auxin in and out of cells and thus regulate auxin polarity transport.Studies have shown that environmental stress can cause the stress response of auxin polar transport,change the downstream regulat ion of PINs protein,and then affect the plant's stress tolerance.In the previous study of the team,it was found that multiple PIN family members of tomato had significant responses to drought and salt stress,and the silencing of Sl PIN1 reduced the drought resistance of tomato plants,indicating that this family member was closely related to the drought resistance and salt tolerance.In order to further understand the response mechanism of Sl PIN gene families under two kinds of adversity and screen th e genes that has the adjustment ability,we analyzed the network of response regulation of tomato Sl PIN family and studied the function of drought resistance and salt tolerance of some family members based on the transcriptome data of tomato response to drought and salt stress in this work,which will lay a foundation for the mechanism research of drought resistance and salt tolerance o f tomato and the practice of resistance breeding.The main findings are as follows:1.Based on transcriptome data of tomato response to drought and salt stress,bioinformatics analysis was conducted.GO analysis showed that Sl PIN family members were mainly involved in cell membrane related and auxin transport-related functions,but there is no gene enriched in any2.KEGG pathway.3.In the transcriptome data for members of Sl PIN interactions associated gene analysis,146 associated genes were obtained,GO analysis showed that these genes not only involved in auxin transport,but also related to iron exchange,DNA phosphorylation and MAPK related functions.KEGG analysis show that these genes are mainly involved in plant hormone signal transduction pathways,nitrogen metabolism pathway and so on.4.Through the analysis of the interaction network of 146 associated genes,4 interaction complex modules were obtained,and 9 important associated genes with more than 10 interaction factors were further screened and obtained,all of which were enriched in the auxin signaling pathway.5.q RT-PCR was performed to verify the expression patterns of the five important associated genes under drought stress and salt stress,and the results were consistent with the transcriptome data.6.After ABA induction,it was found that almost all the members' expressions were up-regulated,among which,Sl PIN3 showed a slow decline after up-regulation under the influence of ABA.7.VIGS gene silencing technology was performed on Sl PIN3,Sl PIN6 and Sl PIN7,respectively,and the results showed that the drought tolerance of plants decreased after the silencing of the three genes.Silencing Sl PIN3 and Sl PIN6 genes could lead to the decrease of the salt-tolerance of plants,while silencing Sl PIN7 gene had no effect on the salt-tolerance of plants. |
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