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Antennal Transcriptome Analysis And Functional Characterization Of Pheromone Receptor Candidates In Codling Moth Cydia Pomonella

Posted on:2021-01-22Degree:MasterType:Thesis
Country:ChinaCandidate:K TianFull Text:PDF
GTID:2393330602990433Subject:Agricultural Entomology and Pest Control
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Insect is the most successful animal on the earth who uses its specific and sensitive olfactory system to conduct important behaviors,such as locating mating partners,food resources,ovipostion sites and avoiding predators.Lepidopteran insects use sex pheromones to communicate between sexes.Olfactory communication and mate-finding can be disrupted and prevented with the synthetic pheromones.Pheromone mediated mating disruption has become a commercially viable pest management technique and is used to control the codling moth,Cydia pomonella,a serious apple pest,on 160,000 ha worldwide.Codling moth is an important pome fruit pest all over the world and also a serious invasive species in China.With the increase of resistance to various insecticides,the technology of mating interference using sex pheromone plays an increasingly important role in the control of codling moth.Although this technique is widely used in pest control,the chemical sensing mechanism of the sex pheromone is still unknown.It is useful to understand the function of odorant receptor,ORs,to data the efficient pest control technology.We will conduct study with molecular biology,electrophysiological techniques and bioinformatics to identify number of the protein genes and functionally analyzed the olfactory receptors.The main results are following:1.Antennal transcriptome analysis and identification of chemosensory genes in codling moth Cydia pomonella.To understand the olfactory mechanisms of codling moth,we conduct transcriptome analysis of antennae by Illumina HiSeqTM 2000 and identified 66 ORs,26 IRs,14 GRs,35 OBPs,16 CSPs,2SNMPs.Moreover,there are 14 PRs in our study including two novel PRs.The result of antennal transcriptome analysis will provide more information for functional study in PRs.2.Functional characterization of pheromone receptor candidates in codling moth Cydia pomonella.Four PRs which contain full-length of open reading frame?CpomOR1,CpomOR2a,CpomOR5,CpomOR7?and four of these with ligands that had not been reported?CpomOR1,CpomOR2a,CpomOR5,OR7?were selected to deorphanize in the heterologous Xenopus oocyte expression system.Both CpomOR2a and CpomOR5 specifically responded to?E,E?-8,10-dodecadien-1-yl acetate.Furthermore,we found CpomOR5(EC50=1.379×10-8 mol/L)was much more sensitive to E8,E10-12Ac than CpomOR2a(EC50=1.663×10-6 mol/L).Since E8,E10-12Ac is an important component in C.pomonella sex pheromone,our results improve the current understanding of pheromone communication in codling moth and will also be helpful for development of pest management strategies.The above results not only help us to understand the complex molecular neural mechanism of sex pheromone communication in odling moth,but also provide a theoretical basis for screening new and effective behavioral regulators and developing new green prevention and control technologies.
Keywords/Search Tags:Cydia Pomonella, Antennal Ttranscriptome, Pheromone receptor, Xenopus oocyte
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