Screening And Research Of Camelina Sativa Mutants Adapted To Subtropical Environment

Camelina sativa belongs to the Brassicaceae family.which is a new oil crop with high economic value.Camelina sativa can grow on arid and barren land,it has excellent agronomic characteristics such drought resistance,pest resistance,saline-alkali tolerance.There are many mountainous hills areas in Zhejiang Province,and the soil is mainly red yellow soil,which is relatively poor.The area unsuitable for crop growth is large.Zhejiang Province is a subtropical region with high temperatures in summer.Camelina sativa is temperate crop and have not been successfully introduced into subtropical regions.This topic uses mutagenesis breeding techniques to cultivate Camelina sativa plants adapted to the subtropical environment.The main result are as the following:1.In this study,Camelina sativa variety Yanshi-1 was used as the experimental material.We used chemical mutagenesis and physical mutagenesis methods,10 000 Camelina sativa seeds were mutagenized to construct a Camelina sativa mutant library.Plant the mutant M1 generation seeds in the field in Zhejiang,and screen the M2 generation mutants by using the subtropical environmental conditions in Zhejiang summer.Finally,three subtropical summer tolerant Camelina sativa mutants were screened and named as subtropical summer tolerant(sst).2.The three sst mutants showed a late flower phenotype,and the expression of the gene FLOWER LOCUS C,which inhibits plant flowering,increased.Through high temperature treatment at 40℃ for 3 hours,we found that sst is more heat resistant than wild type.Transcriptome analysis found that the up-regulated genes were significantly enriched in redox and stress-related areas.Hydrogen peroxide staining and pathogen infection proved that the sst mutant produced less hydrogen peroxide and increased resistance to pathogens under heat stress.3.We researched the impact on Camelina sativa regeneration capacity of many factor as explant,plant hormone.The result showed that the best explant material is the hypocotyl part of the plant.It can produce callus under 9 kinds of callus-inducing hormone ratio schemes,with a callus induction rate of 99%.In summary,we have successfully obtained Camelina sativa mutants adapted to the subtropical climate,and proved that the increased heat resistance of the mutants is related to the increased ROS clearance of the mutants.This topic points out that the introduction of temperate crops to subtropical regions should not only improve their heat resistance,but also have the ability to resist pathogens.In this way,temperate crops can adapt to the high temperature and high humidity environment in the subtropical region.