Bioinformatics Research Of Genome Structure And Function Evolution In Camelina Sativa

Camelina sativa is an emerging oilseed crop with several advantages of high production and resistance to drought and diseases.Accomplishment of whole-genome sequencing of C.sativa provided the data for studying on the structure and function evolution of the C.sativa genome.In this study,we respectively drew the homologous gene dot-plots within the genomes by taking grape,model plant Arabidopsis thaliana and Brassica plant Brassica rapa as out-group reference to verify that C.sativa represents a whole-genome triplication event relative to A.thaliana.By characterizing the homology structure between A.lyrata and C.sativa chromosomes,we inferred ancestral diploid karyotype of C.sativa,including 7 ancestral chromosomes,and reconstructed the evolutionary trajectories leading to the formation of extant C.sativa genome based on the telomere-centric model.The process involves 2 chromosome fusions.By searching synteny regions within genomes,we construct collinearity lists with grape and A.lyrata as reference to generate global and local homology combination alignment map of genomes within multiple genomes.By analyzing the retain and loss of homology genes based on the collinearity list,we found that the level of retain of homology genes among the three sub-genomes had no significant difference.We identified 1675 lipid metabolism related genes in C.sativa and found that 1411 genes were generated by WGT of C.sativa and 83 genes were generated by tandem duplication.By identifying and analyzing ACC genes which played an important role in the process of fatty acid synthesis,we did not detect any positive selection sites.The result provided important resources in comparative genomics of C.sativa,especially in the aspect of deeper understanding the formation and evolution of C.sativa chromosomes.Figure 21;Table 7;Reference 58...