Identification Of Tick Species On The Surface Of Bactrian Camel And Isolation Of Tick Pathogens In Alxa,Inner Mongolia

The diversity of ecological environment and animal species provide suitable conditions for many ticks to survive in Inner Mongolia.In recent years,the mutation of global climate stimulates the frequency of tick activity and the number of ticks,which is difficult to control.This is followed by many tick born diseases including some zoonose.Materials and Methods:385 ticks were collected from the body surface of camels in three areas of Ejina Banner,Alashan.First of all,the ticks were identified by traditional morphological methods and molecular biological methods.Then,the gene polymorphism of 16S rRNA was analyzed by genetyx version 6 software.In addition,we detect tick pathogens using specific primers by PCR.Finally,sequence amplification,sequencing and phylogenetic tree analysis were used to identify the pathogens.Further,we isolated rickettsia from ticks by Vero cells.Results:The morphology of ticks collected in this study was in accordance with the typical morphological characteristics of H.asiaticum.The results fo molecular biological identification showed that the tick was in the same evolutionary branch with H.asiaticum in Xinjiang,China;three sequences of 16S rRNA gene showed different degree of gene polymorphism with the other 27 genome sequence of ticks.In the results of pathogen detection,18 ticks(4.6%)and 5 ticks(1.3%)were infected by Rickettsia and Coxiella respectively.We also found the same tick could be infected by two kinds of pathogens.NCBI-BLAST analysis and phylogenetic tree analysis show that Richettsia IMA-1 was an unidentified species,which belongs to spotted fever Rickettsia.In addition,C.burnetii IMA-1 and Coxiella burnetii str.rsa439(GenBank:cp040059.1)belong to the same class.In addition,Rickettsia was successfully isolated from ticks in Vero cells cultured at 28?.Conclusion:All 385 ticks collected in this study were identified as H.asiaticum,and there were genetic polymorphisms in the tick.Two pathogens were detected,one undetermined species Rickettsia of spotted fever,the other was Coxiella burnetii,which was the pathogen of Q fever,and Rickettsia was successfully isolated.