Role Of Extracellular Calcium In Ethanol Activation Of Mouse Oocytes

In mammals,ovulated oocytes are arrested at the metaphase stage of the second meiotic division until fertilization or given physical and chemical stimulation.The restoration of meiosis is called"activation"and is characterized by the formation of polar bodies and male and female pronuclei.The activation of mammalian oocytes at fertilization or at parthenogenetic activation is always associated with intracellular Ca²⁺oscillations.This experiment investigates the effect of extracellular calcium on the activation rate of alcohol-stimulated mouse oocytes with extracellular calcium during in vitro aging process of oocytes and during the culture process after alcohol stimulation.Combining the inhibition of L-type calcium pathway and calcium sensing receptor to observe the effect of extracellular calcium on alcohol-stimulated activation of mouse oocytes.Look for ways to increase the parthenogenetic activation rate of mouse,and provide technical support for other animal reproduction biotechnologies,such as animal cloning,somatic cell nuclear transfer,animal genetic engineering,and embryonic stem cells.The result shows:?1?There was a significant increasion in activation rate?79.7%VS 6.8%,P<0.05?of mouse oocytes after ethanol activation.?2?There was a significant increasion in ethanol activation rat?72.5%VS 14.2%,P<0.05?of mouse oocytes with extracellular calcium during in vitro aging;There was a significant increasion in ethanol activation rat?48.1%VS 14.2%,P<0.05?of mouse oocytes with extracellular calcium during culture process after alcohol stimulation.?3?There was a significant reduction in ethanol activation rat?55.4%VS 82.3%,P<0.05?of mouse oocytes with 2?M NPS-2143 during in vitro aging;There was a significant reduction in ethanol activation rat?39.0%VS 47.5%,P<0.05?of mouse oocytes with 2?M NPS-2143during culture process after alcohol stimulation.?4?There was a significant reduction in ethanol activation rat?11.8%VS 82.3%,P<0.05?of mouse oocytes with 400?M Nifedipine during in vitro aging;There was a significant reduction in ethanol activation rat?30.9%VS 47.5%,P<0.05?of mouse oocytes with 400?M Nifedipine during culture process after alcohol stimulation.?5?There was a significant reduction in ethanol activation rat?4%VS 82.3%,P<0.05?of mouse oocytes with 2?M NPS-2143 and 400?M Nifedipine during in vitro aging;There was a significant reduction in ethanol activation rat?18.9%VS 47.5%,P<0.05?of mouse oocytes with 2?M NPS-2143 and 400?M Nifedipine during culture process after alcohol stimulation.This experiment studies the role of extracellular calcium in the activation of mouse oocytes,and found that extracellular calcium plays an important role in both in vitro aging and culture process after alcohol stimulation..Calcium sensing receptors and L-type calcium pathways have an important effect on the activation of mouse oocytes stimulated by alcohol.With extracellular calcium during the in vitro aging and culture process after alcohol stimulation can significantly increase the activation rate of mouse oocytes.