| Stellaria dichotoma L.var.lanceolata Bge is an important medicinal plant in China,which found in Ningxia are high in medicinal value and are a prime source of the Chinese medicine Yinchaihu.With the shortage of wild Stellaria dichotoma,the artificial planting area is being promoted year by year,and the demand for seeds in production is increasing.However,the mixed provenances,uneven quality,and many fakes of the seeds provided on the market have seriously affected the quality and yield of Stellaria dichotoma medicinal materials.The aim of this study is to provide provenance material with high content of medicinal ingredients,good seed quality and high resistance for genuine producing area by studying for seed quality,seedling stress resistance,and medicinal material activity of 13 materials.The results show that:1.There are differences in germination potential,germination rate,seed viability,and thousand seeds weight of Stellaria dichotoma seeds from 13 provenances.The optimum germination temperature of Stellaria dichotoma seeds is 20??25?;85%of the seeds have a viability of more than 95%,and 62%of the seeds have a thousand seeds weight of 1.45?1.65g.Based on membership function and cluster analysis,13 provenances are divided into four categories:good quality—No.10,No.5,No.13,and No.7,and followed by—No.2,No.9,and No.4,and medium quality—No.11,No.l,No.6,No.3,and No.8,and poor quality—No.12.2.The MDA content,membrane permeability,CAT activity,SOD activity,POD activity,Pro content,and soluble protein content of different Resistance indexs of Stellaria dichotoma from 13 provenances were different in the first and second years.Based on cluster analysis,13 provenances are divided into three categories:strong resistance—No.3,No.9,No.12,and No.4,which mainly come from Tongxin and Hongsibao area in Ningxia,and medium resistance—No.2,No.13,and No.8,which mainly come from Tongxin in Ningxia and Longxi in Gansu,and weak resistance—No.6,No.7,No.1,No.5,No.11,and No.10,which mainly come from Tongxin and Pengyang area in Ningxia.3.The content of annual flavonoids in Stellaria dichotoma is 2.27?3.17mg·g-1,and the content of biennial flavonoids is 3.85?8.19 mg·g-1.The content of annual total saponins in Stellaria dichotoma is 34.75?42.56 mg·g-1,and the content of biennial total saponins is 38.22?45.17 mg·g-1.Based on the contents of total flavonoids and total saponins,the Stellaria dichotoma with high content of medicinal ingredients of provenance No.3,No.12,No.l,No.4,No.2,and No.6 were screened.The yield of biennial Stellaria dichotoma is 120?205 kg/667 m2,and the higher-yielding provenances No.9,No.5,No.6,and No.12 come from Tongxin area in Ningxia,and among them,the provenances of No.6 and 12 are also high medicinal ingredients.4.Principal component analysis was performed on 13 provenances of Stellaria dichotoma quality indicators such as medicinal components,resistance,and seed quality,and the component score coefficient results show that principal component 1 reflects the indicators of Pro,germination rate,and viability component,and principal component 2 reflects the quality indicators of total flavonoids,CAT,and thousand seeds weight,and principal component 3 reflects the quality indicators of total saponin and SOD,and principal component 4 reflects the quality indicators of MDA.According to the comprehensive score of each provenance,the quality of various provenances of Sterllaria dichotoma was in turn:No.4>No.1>No.10>No.5>No.2>No.7>No.8>No.3>No.6>No.11>No.9>No.13>No.12,and the top comprehensive score are No.4,No.1,No.10,No.5,indicating that these four provenances of Stellaria dichotoma have better quality. |
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