Study On The Mechanism Of ABCG Transporter Against Abiotic Stress In Cajanus Cajan

The sustainable management of forest resources is the basic approach and criterion to realize the sustainable development of forestry,and it is also the ultimate goal of forest resource management.Improving the development and research of forest resistant species resources is an important direction and breakthrough point for realizing the sustainable development of forestry,and it is also an important measure to realize forest resource management.Pigeon pea(Cajanus cajan)is the only woody edible legume crop in the world,and also is one of the important food crops in southern China.It is also a particularly important plant resource for the sustainable development of forest resources.However,the long-term exposure of pigeon pea to the hot and dry environment and the erosion of acidic and heavy metal soil environment not only severely affected the yield and quality of pigeon pea,but also affected the ecological value of pigeon pea in maintaining water and soil.ABCG transporters(ATP-binding cassette transporters subfamily G)is a family of proteins widely present in plants,plays an important role in the efflux of heavy metal ions in plants and resists drought stress and other physiological processes.Therefore,the ABCG transporters were scientifically identified in pigeon pea in this study,and explored and discussed the important role of ABCG transporters resisting abiotic stress in pigeon pea,to promote the research and cultivation of pigeon pea resistant varieties and promote the sustainable development of forest resources.The main conclusions of this paper are as follows:1.Identification and structure analysis of pigeon pea ABCG transporter.In this study,51 ABCG transporters were identified in the whole genome of pigeon pea;chromosome location analysis showed that the ABCG gene of pigeon pea is located on other chromosomes except chromosome 7,and there are tandem duplications;phylogenetic analysis showed that ABCG transporters have evolved highly conserved,but divided into two subgroups: PDR transporter(Pleiotropic drug resistance)and WBC transporter(White brown complex).The basic physicochemical properties and structural function analysis of the protein showed that the ABCG transporter domain of pigeon pea is highly conserved,but the two subgroups have obvious differences in structure.The WBC transporter has a transmembrane domain and a nucleotide binding domain.The PDR transporter has two transmembrane domains and two nucleotide binding domains.2.Expression analysis of pigeon pea ABCG transporter gene.The gene structure and upstream regulatory elements of pigeon pea ABCG gene were analyzed in this study,and found that there are more exon structures and upstream stress response regulatory elements,which will significantly affect the expression of ABCG gene in response to changes in the external environment.The ABCG gene of pigeon pea is highly expressed in roots and leaves,which further showed that the ABCG transporter plays a role in the resistance of pigeon pea in roots and leaves.In the simulated abiotic stress,the ABCG transporter gene has obvious expression differences: Cc ABCG28 responds to low temperature stress,Cc ABCG19 responds to drought and aluminum stress,and Cc ABCG7 is significantly up-regulated under high temperature stress.In summary,this study identified the ABCG transporter in pigeon pea,analyzed the basic physical and chemical properties,structure and function of the ABCG transporter,and further analyzed the expression of the ABCG gene of pigeon pea under simulated abiotic stress.These research results confirm the importance of pigeon pea ABCG transporter in resisting abiotic stress,broaden the study of related genes in the resistance molecular mechanism of pigeon pea,and lay the foundation for the cultivation of excellent pigeon pea varieties and promote the development of forest resources.Continuous development provides new options.