Assessment Of Genetic Diversity Of Pigeonpea(Cajanus Cajan(L.)Millspaugh) Germplasm Resources

Pigeonpea (Cajanus cajan(L.) Millspaugh) is one of the important grain legume crops in the tropics and subtropics. The objective of this work is to find the relationships among classification, geographical distribution and evolution of pigeonpea based on diversity analysis, in order to generate information for the following up researches.The results of the analysis on morphological characters showed that pigeonpea were sensitive to different ecological conditions. Ecological issues should be considered if the introduction of pigeonpea varieties occurred between different regions. The average CV was 0.319, Shannon-Weaver index was 1.066. The correlations among days to flowering, days to mature, plant height and number of branches were significantly positive. The top 5 principal factors totally represented 72.813% of morphological diversity. Based on morphological data, 77 accessions were clustered into 3groups.AFLP finger printing system was optimized by employing of 2 accessions of pigeonpea (Cajanus cajan) and 1 accession of wild pigeonpea (Cajanus scarabaeoides), for genetic diversity analysis. 20 pairs of informative primers were identified and recommended for AFLP analysis to reveal genetic diversity of pigeonpea.A representative set of 139 pigeonpea(Gyanus cajan) genetic resources from Asia, Africa and Latin America, together with an out group standard wild pigeonpea (Cajanus scarabaeoides), were analyzed by amplified fragment length polymorphism(AFLP) methodology, using 17 informative primer pairs. In total, 502 unambiguous bands were created, of which 494 were polymorphic, about 98%. According to the dendrogram by UPGMA cluster analysis using Nei's(1972) distance calculations based on AFLP banding, all the accessions were distinct and revealed enough genetic diversity for identification and classification of accessions within Cajanus cajan. From ME cluster analysis, 9 distinct evolutionary groups were detected. The preliminary results indicated that China maybe one of the pigeonpea genetic diversity minor center.Distinct and unique bands between sterile line (A line) and maintainer line (B line) of CMS were detected in the study, which may be potentially useful on marker assisted hybrid breeding and heterosis exploration of pigeonpea.