Study On Leaf Phenotype And SCoT Genetic Diversity Of Six Species Of Scet.chrysantha

Golden Camellia is one of the important camellia plant resources in Guangxi,and is famous for its unique yellow color and its rarity.Due to the large-scale man-made influence and destruction in recent years,the native population of golden Camellia has been greatly reduced,which has put tremendous pressure on the protection of plant resources.In this study,using paraffin wax and SCoT molecular markers,respectively collected 6 species of golden Camellia,including native species(from Nonggang Nature Reserve)and cultivated species(from Nanning Golden Camellia Park)of C.chrysanthoides,C.impressinervis,C.limonia,C.longgangensis,C.ptilosperma,C.perpetua,the leaf phenotype and molecular genetic diversity were analyzed,and the influence of interspecies and habitat differences on its genetic diversity was compared to provide a reliable theoretical basis for the introduction,cultivation and protection strategies of golden Camellia resources.The study results are as follows:1.Golden Camellia in different habitats has large differences in the outer shape of the leaves,but there are relatively few differences in anatomical morphology.The phenotypic traits of golden Camellia leaves are widely dispersed,with significant variation and abundant genetic diversity.Variation among populations is the main source of population diversity,and cultivars have a higher degree of variation.Only from the perspective of inter-species variation of golden Camellia,the richness of diversity is as follows: C.impressinervis > C.limonia > C.chrysanthoides > C.perpetua > C.ptilosperma > C.longgangensis.It shows that geographical and climate factors can have a certain degree of influence on phenotypic variation.2.The principal component analysis mainly reflect the differences in the phenotypic traits of golden Camellia: leaf width,leaf anatomical thickness,phloem thickness and Palisade tissue-spongy tissue ratio.3.Based on the clustering of leaf phenotypic traits,12 parts of golden Camellia germplasm are grouped into two groups.The first group includes C.chrysanthoides,C.impressinervis,C.ptilosperma,C.longgangensis;the second group includes C.limonia and C.perpetua.Cultivated species clustering is different from native species clustering.Based on the clustering of SCoT molecular markers,12 parts of golden Camellia germplasm are grouped into two groups.The first group includes C.impressinervis,the second group includes five other species of golden Camellia.The phenotypic clustering results of golden Camellia are different from those of molecular marker clustering.Because the shape and structure of plants are easily affected by the environment,the results of molecular marker clustering of golden Camellia are more reliable,the phenotypic trait clustering result section is used to assist identification.The phenotype and SCoT cluster of golden Camellia had no correlation with geographical distance.4.The six species of golden Camellia had high genetic diversity at the molecular level,and the richness of genetic diversity is relatively high,but there is little difference in the richness of genetic diversity among species.Gene communication between populations is limited,genetic differentiation is high,and genetic variation mainly exists among populations.Therefore,as much as possible for the native species to protect its population and protect the environment where it grows,cultivated species can expand the number of plants by cutting and other methods.