Effects Of Yeast Glycoprotein On Growth Performance And Intestinal Barrier Function In Weaned Piglets

Yeast glycoprotein is a substance with antibacterial activity that is released after directed enzymatic hydrolysis of yeast cells.In this study,we aimed to evaluate effects of yeast glycoprotein and antibiotics on growth performance and intestinal barrier function of weaned piglets.In this study,weaned piglets were feed with yeast glycoprotein instead of antibiotics.A total of 180 of weaned piglets(with day 23±2 days)of age were selected and assigned to two groups randomly divided into 2 groups: Antibiotics group(feed a basal diet with 800 g/t Enduracidin and 200 g/t Quinocetone)and yeast glycoprotein group(feed a basal diet with 800 g/t yeast glycoprotein),each group containing 6 replicates and 15 pigs per replicate.These was added to the basic diet for 3 days in advance and 14 days in the formal trial period.Growth performance,diarrhea rate and organ index were recorded,in addition,intestinal permeability and intestinal barrier function were observed.The results showed that:1)The average daily gain,final weight and feed weight ratio of piglets in yeast glycoprotein group were higher than those in antibiotic group(0.05 < P < 0.01).There were no significant differences in organ index,diarrhea rate and diarrhea index between two groups(P >0.05).2)Compared with the antibiotic group,yeast glycoprotein significantly decreased the serum DAO activity of piglets(P <0.05).3)Compared with the antibiotic group,yeast glycoprotein decreased significantly VH in jejunum and CD in jejunum in ileum of piglets(P <0.05),in addition,VH and V/C in ileum were increased significantly in yeast glycoprotein group(P <0.05).4)Compared with the antibiotic group,yeast glycoprotein significantly increased the m-RNA expression level of Occlaudin protein in duodenum of piglets(P <0.05).In addition,yeast glycoprotein had a tendency to increase m-RNA expression level of ZO-1 protein in the duodenum and Occlaudin protein in the jejunum(0.05< P <0.10).5)Compared with the antibiotic group,yeast glycoprotein increased significantly m-RNA expression level of Hsp-70 protein in duodenum and ileum(P <0.05).In addition,yeast glycoprotein decreased significantly the m-RNA expression levels of IL-12 protein in duodenum and Hsp-70 protein in jejunum(P <0.05).6)At the order level,the relative abundance of Bacillales and Gastranaerophilales in the yeast glycoprotein group was significant lower than those in the antibiotic group(P <0.05).At genera level,the relative abundance of colonic bacteria Blautia in yeast glycoprotein group was significant lower than that in antibiotic group(P <0.05),and the relative abundance of colonic bacteria Lactobacillus in yeast glycoprotein group was significant higher than that in antibiotic group(P <0.05).In conclusion,compared with antibiotics,yeast glycoprotein can improve growth performance and reduce diarrhea rate by improving intestinal morphology,up-regulating m-RNA expression of intestinal mucosal tight junction protein and immune related genes,optimizing colon flora abundance and diversity.Therefore,antibiotics can be replaced by yeast glycoprotein to a certain extent.