The Mechanism Intervening Mice Lewis Lung Cancer Metastasis With Deficiency Of Spleen-QI Based On The Expression Of Nm23 And CD44

Objective:To investigate the the mechanism of Huangqi Jianzhongtang(HQJZT)on spleen deficiency syndrome and mice lung cancer inhibiting tumor metastasis.Method:The purgation of the bitter cold diarrhea in mouse to establish spleen deficiency syndrome Lewis lung cancer model,the model is successfully established,accord ing to the experimental design to handle all of the mice while observing the overall situation of the mice.14 days after the eye to take blood,serum xylose level after 28 days,thymus,lung and tumor tissue.,Respectively,said take each mouse thymus weight,weight,number of lung metastatic nodules and the number of calculation of the thymus index,using RT-PCR method for detection of nm23expression of CD44.Results:The model of Lewis lung carcinoma in mouse spleen deficiency syndrome,the overall state of the spleen deficiency in mice and decreased immune function,high doses of Huangqi Jianzhongtang(HQJZT)can significantly improve the overall situation of the mice spleen deficiency syndrome and Lewis,increased the thymus index.nodules less than the model group;high dose group metastasis rate was 36.4%;middle dose group metastasis rate was 40.9%;low dose group metastasis rate was 32.8%;huangqijianzhongtang(HQJZT)each dose group can decrease the expression of CD44 expression and increase nm23.Conclusion:Deficiency of spleen qi could suppress immune function of mice,and promote the growth and metastasis of the cancer.High dose Huangqi Jianzhongtang(HQJZT)has a certain degree of inhibition of the transfer of spleen deficiency syndrome and lung tumors in mice,improving the overall situation of the mice,enhance thymus index,decrease the expression of CD44 and increase the expression of nm23's.The main mechanisms may be relate to such factors as below:(1)improving the whole condition of the mice,enhancing their thoracic gland index;(2)raising the level of xylose in blood-serum to improve the condition of deficiency of spleen qi;(3)up-regulating the level of nm23;(4)down-regulating the level of CD44;(5)inhibiting tumor growth and metastasis.