Preliminary Study On Anti-SUMO Antibody In Multiple Autoimmune Diseases

Objective:In this study,we constructed a plasmid,specifically expressed SUMO protein,and to study the expression level of SUMO protein in the serum of patients with primary biliary cholangitis,systemic lupus erythematosus,rheumatoid arthritis,and sjogren's syndrome which were typical of autoimmune diseases.Explored whether anti-SUMO antibodies could serve as specific serum markers for PBC.Combined with the patient's clinical records,the intrinsic link between SUMO and Sp100 and the impact on liver function of patients were explored.Methods:1.The plasmids containing SUMO1,SUMO2 and SUMO3 fragments were prepared by PCR and ET cloning technique,and then introduced into E.coli for protein induction and expression.The resulting proteins were finally purified.2.The spot-blotting method was used to preliminarily screen SUMO-positive specimens from serum samples of patients with primary biliary cholangitis(PBC),and Western blot was used to verify the results.Through continuous improvement of experimental conditions,an optimal ELISA diagnostic system for anti-SUMO antibodies was established.3.The positive rate of three subtypes of anti-SUMO antibody in PBC,SLE,SS,and RA were detected by the established ELISA diagnostic system,and their differences were analyzed by chi-square test.Dot blotting and Western blot verified the validity of the ELISA diagnostic system.4.Combine the patient's clinical history data,and use the t-test to perform statistical analysis on the various biochemical indicators of the patient.Find the commonalities of illness in patients with anti-SUMO antibody positive,and then separate the autoimmune diseases from SUMO1,SUMO2 and SUMO3 Subtypes,in order to facilitate the later study of its pathogenic mechanism,provide basis for providing targeted treatment.Results:1.Compared with other non-PBC autoimmune diseases and healthy controls,the positive detection rates of anti-SUMO1 antibody,anti-SUM02 antibody,and anti-SUM03 antibody in PBC were high(P<0.01).2.The specificity of anti-SUMO antibody markers in PBC was as high as 99%,and its sensitivity remained at around 86%.3.There was no significant difference in the positive rate of anti-SUMO antibody among other autoimmune diseases(P>0.05).4.In the anti-Sp100 antibodies positive and negative PBC samples,the positive rate of anti-SUMO antibodies in the anti-Sp100 antibody positive group was significantly higher than that in the anti-Spl00 antibody negative group(P<0.05),and the mean total bilirubin and direct bilirubin values were significantly higher than those with anti-SUMO antibody negative group(P<0.05).5.The bilirubin mean value was higher in other autoimmune diseases in the anti-SUMO antibody-positive group than in the anti-SUMO antibody-negative group.Conclusion:The highly specific anti-SUMO antibody is expected to become a novel antibody diagnosed by PBC,which is of great significance for improving the clinical diagnosis efficiency of PBC.The SUMO protein is closely related to Sp100,which has a potential impact on the patient's liver function.