The Effects Of Brain Derived Neurotrophic Factor On Learning And Memory Function Of Post-traumatic Stress Disorder Model Rats

Objective: To investigate the effects of brain-derived neurotrophic factor and TrKB receptor on the learning and memory function of rats with post-traumatic stress disorder.Methods: The rats were randomly divided into six groups,including SPS Model and 7,8-dihydroxy flavone low dose group(SP+D1,n=6),SPS Model and 7,8-dihydroxy flavone high dose group(SP+D2,n=8),SPS Model and Sertraline group(SP+SER,n=7),SPS Model and Normal Saline group(SP+NS,n=7),SPS Model group(SP,n=8),Control group(C,n=7).Except for the normal saline group,all the rats in other five groups were modeled by single-prolonged stress(SPS)to induce post-traumatic stress disorder(PTSD).After the model rats were prepared,all rats were tested by open field tests and elevated cross maze tests for behavioral identification.Thereafter,the rats in the normal group and the model group undertook no more treatment;the rats in the SP+NS Group were injected normal saline for 14 days(2 ml/kg,ip,qd),while the other rats in 3 groups were treated with the 7,8-dihydroxy flavone(5 mg/kg,10 mg/kg,ip,qd)and Sertraline(10 mg/kg,ip,qd)for 14 days.Finally,after the treatment,the open field test,elevated plus-maze test were conducted again,and Morris water maze(MWZ)test was also performed to all rats.Results:(1)The analyses of open-field test results: The open-field test of 6 groups before drug intervention showed that,the total distance and the stopover time in the central region of the model rats were less than that of the C group(P<0.01).After drug intervention,compared with SP Group,the total movement distance of rats in SP+D2 Group(P<0.01),SP +SER Group(p<0.05)and C Group(P<0.01)increased obviously,the number of exploration of rats in SP+D2 Group(P<0.01),C Group(P<0.01)also increased significantly.(2)The elevated plus-maze test results: The open arm entry frequency of model rats was less than the C group(P<0.05)before the drug intervention.After drug intervention,compared with SP Group,The open arm entry frequency in SP+D1 Group(P<0.05),SP +D2 Group(P<0.05),Group C(P<0.01)increased significantly,the open arm retention in time of SP+D1 Group(P<0.05),SP +D2 Group(P<0.05),SP+SER Group increased significantly.(3)The analyses of Morris water maze test results: The escape latency of rats in 6 groups in the place navigation test during 7 test days showed that,test days(P<0.01)and animal groups(P<0.01)were significantly different,and on the 4th,5th,7th day of the test process,the main effect between groups were significantly different.Significant differences of rat escape latency between the SP+D1 Group and the SP Group on the sixth day(P<0.05),and between SP+D1 Group and the SP Group,or SP+ D2 group and SP Group on the seventh day(P<0.01),were observed.In the space exploration experiment,the time of the first time to reach the platform of rats in SP+D2 Group was shortened obviously in comparison with and SP Group or SP+NS group rats(P<0.01).Conclusion: Brain derived neurotrophic factor and TrKB receptor can improve locomotor activity,working memory and learning function in PTSD rats induced by the SPS method.