Detection And Significance Of S1P In Cerebrospinal Fluid Of Children With Viral Encephalitis And Suppurative Meningitis

Background and ObjectiveViral encephalitis(VE)and purulent meningitis(PM)is the most common childhood children with intracranial disease.Children's own development of central nervous system is imperfect,low immunity,blood-brain barrier is weak,pathogens easily through the blood-brain barrier into the brain,causing intracranial infection,leaving sequelae,and even death,a serious threat to children's lives and health.Viral encephalitis(VE)is a common disease of acute intracranial inflammation caused by a variety of viral infections.In recent years,the incidence increased significantly,acute onset,rapid progression.If not treated in time will seriously endanger the lives of patients or legacy nervous system sequelae.Purulent meningitis(PM)is a serious infectious disease in children with intracranial infection,easy to misdiagnosis,variable symptoms,and the use of antibiotics unreasonable and irregular increase atypical features of PM,CSF-related detection and pathogen detection Lower rate,an increase of purulent meningitis diagnosis.Early identification of both diseases,diagnosis,comprehensive treatment for the outcome of the disease and the incidence of complications have a certain impact.Recent studies have shown that the S1P(sphingosine-1-phosphate)signaling pathway is involved in the physiological and pathological processes of immune system diseases,cardiovascular diseases,tumor pathogenesis,and the like,while the role of the nervous system and inflammatory diseases is gradually gaining attentions.However,the research on intracranial infection in children has not been reported yet.In this paper,enzyme-linked immunosorbent assay(ELISA)quantitative measurement of S1 P in children with brain disease and brain changes in CSF,to explore its mechanism of action.Methods30 cases(19 males and 11 females,4.2 ± 2.2years)which diagnosed as viral meningitis disease were selected were selected from April,2016 to April,2017,who were diagnosed as purulent meningitis.At the same period,30 children(18 males and 12 females,3.7 ± 1.6years);During the same period of hospitalization,20 patients without CNS infection and immunodeficiency were included as controls group(12 males and 8females,3.6±1.8 years).There were no significant differences in sex,age,and weight between the three groups(P> 0.05).All the 3 groups were lumbar punctured within 24 hours after admission.5ml CSF was collected,2ml in sterile EP tube,placed at-80 ? environment waiting for check,3ml were sent to the laboratory for biochemical,routine,staining.The levels of S1 P in CSF were measured by enzyme-linked immunosorbent assay(ELISA).At the same time consult all the subjects of the relevant cases,collect clinical data,analysis.Use SPSS21.0 statistical software package for data analysis,With ? = 0.05 for the test criteria.Result1.The levels of S1 P in the CSF of in PM group,in VM group and con trol groups were(0.217±0.017)nmol/L,(0.191±0.010)nmol/L,and(0.172±0.015)nmol/L,respectively.There was a statistical difference between each group(P<0.05).The level of S1 P in the CSF of the PM group was higher than th e VM group and the control group.The level of S1 P in the CSF of the PMgroup was higher than that of the control group.2.S1 P in cerebrospinal fluid was correlated with N-XP,GLU,ADA,CSF-P,LDL and CL Sexual analysis.The results showed that S1 P in CSF was positively correlated with cell number,negatively correlated with glucose,and n-o correlation with other indexes.Conclusion1.Purulent meningitis and viral encephalitis in acute cerebrospinal fluid expression of S1 P increased,and purulent meningitis group increased significantly.2.S1 P is involved in the pathogenesis of viral encephalitis and purulent meningitis in children and may have different levels of action in these two diseases.