Influence Of Nage Tongmai Decoction On The Expression Of NF-?B-P65 And I?B-? In Rats With Myocardial Ischemia

Objective:The PCR technique was used to determine the RNA expression of NF-?B-P65 and I?B-? in the ischemic model of Wistar rats after Nage Tongmai decoction treatment,so as to study the possible pharmacological effects of the drug on myocardial ischemia.Methods:90 healthy adult Wistar rats were randomly divided into normal group,model group,Xintong oral liquid positive control group,and Nage Tongmai decoction high,medium and low dose group.The high-fat feed was fed for 4 weeks at a dosage of25g/100g/24 h.The high-fat feed was formulated with 3% cholesterol,2% lard,0.2% propyl thiouracil,0.5% sodium choleate and 76.3% basic feed.Last 3 until the fourth week,normal group gives the intraperitoneal injection of saline,intraperitoneal injection of groups isopropyl adrenaline,daily 1 time,observe the ecg T wave change,preparation of myocardial ischemia model in rats.After 4 weeks of continuous gavage,the gene expression of NF-?B-P65 and I?B-? in the myocardium of rats were semi quantitatively analyzed by PCR technology.Results:Compared with the positive control group and the model group,the expression of NF-?B-P65 gene was significantly reduced,while the expression of I?B-? a gene in the Nage Tongmai decoction was significantly higher than that in the model group.The difference was statistically significant in the high dose group of Nage kappa capsule(P<0.05),In the high-dose group of nag tongmai decoction,the difference was statistically significant(P<0.01).Conclusion:Nalge Tongmai decoction can reduce the expression of NF-?B-P65,promote the gene expression of I?B-? and resist endothelial injury,which may be the mechanism for its treatment of CHD and delayed atherosclerosis.