Fluorescence Labeling Of Adipogenic Mesenchymal Stem Cells And LSK Cells In Vivo Homing Of Mice With Myeloablative Conditioning Regimen

Objective: Fluorescence labeling was used to detect the homing of lipogenic mesenchymal stem cells and LSK cells separated by the combination of magnetic beads and flow.Methods: The ADSCs,BMSCs and RF cells of mice were isolated and cultured in vitro,and LSK cells were separated by the latest magnetic beads and flow separation technology.LSK cells were co-cultured with ADSCs and BMSCs,and the total number of LSK cells was counted at 14 d and 35 d respectively to determine the effect on their proliferation.Using living DID fluorescent markers and CSFE markers,ADSCs and RF LSK cells,respectively,by grouping the tail vein injection of LSK,ADSCs + LSK,RF +LSK qing myeloid pretreatment of balb/c mice,feeding executed after 24 hours,wax block pathological,confocal analysis the distribution of fluorescence labeled cells in mice.Results: the current magnetic beads and flow separation technique can significantly increase the rate of LSK cells obtained,ADSCs cells at 14 d of LSK function below BMSCs cell proliferation,and 35 d ADSCs of LSK cell proliferation significantly higher than that of BMSCs,the difference was statistically significant(p < 0.01).Cell labeling by fluorescent confocal detection mice that ADSCs group compared with single infusion LSK and into a fibrous infusion set,obviously promote the homing of hematopoietic stem cells,which distributed mainly in the bone marrow,liver,lungs.Conclusion: Current magnetic beads and the combination of flow separation technology can obviously improve the LSK cells get rate,ADSCs cells at 14 d of LSK function below BMSCs cell proliferation,and35 d ADSCs of LSK cell proliferation significantly higher than that of BMSCs,thedifference was statistically significant(p < 0.01).Cell labeling by fluorescent confocal detection mice that ADSCs group compared with single infusion LSK and into a fibrous infusion set,obviously promote the homing of hematopoietic stem cells,which distributed mainly in the bone marrow,liver,lungs.Conclusion: the combination of magnetic beads and flow separation sorting LSK technology is superior to the traditional pure flow separation technology,ADSCs long-term support hematopoiesis is better than that of BMSCs in vitro,ADSCs can facilitate the homing of hematopoietic stem cells in the body.