Fluorescence Quenching Immunoassay Was Used To Detect Cardiac Troponin I,Heart-type Fatty Acid Binding Protein In Blood And System Software Development

Objective:Based on background fluorescence quenching immunochromatography and internal filtration effect fluorescence quenching magnetic effect separation immunoassay detection system.The detection method of cardiac fatty acid binding protein and cardiac troponin I was established and used for the clinical serum samples.Compiling microsequence injection-spectroscopic analysis of drug concentration process monitoring software,to realize the real-time in-situ process qualitative and quantitative of samples.Methods:Based on the background fluorescence quenching immunochromatography screening test system gold labeled antibody and capture antibody concentration,establish two substances detection methodology and used for clinical sample detection.Based on the filtering effect detection system,synthesis a kind of can occur within the filtering effect to detect serum samples of fluorescence quenching agent nanometer gold bar,optimize detection system to establish two substances detection method and used in clinical samples.Combining the optical fiber sensing and microsequence injection,compiled an concentration on-line monitoring software of ultraviolet and fluorescence spectra.Results:1.The linear concentration range of cardiac fatty acid binding protein was 2.0-100.0ng/mL,the limit of detection was 1.2ng/mL based on background fluorescence quenching immunochromatography.There was no significant difference compared to immunological transmission turbidimetry(p<0.01,r=0.99),and there was no significant difference between the two methods(p>0.05).The linear concentration range of cardiac troponin I was 0.2-30.Ong/mL,the limit of detection was 0.2ng/mL.There was no significant difference compared to immunological transmission turbidimetry(p<0.01,r=0.95),and there was no significant difference between the two methods(p>0.05).2.As a fluorescence quenching agent,the nanometer gold bar with specific absorption peak was synthesized to optimize the synthesis conditions.The maximum absorption wavelength was 682nm and the length ratio was 1.7.Gold bars mark the antibody of two substances,the antibody activity remains unchanged.The sample pool length of the detection system and fluorescence dye concentration.3.Based on the internal filtration effect fluorescence quenching magnetic effect separation immunoassay detection system,the linear concentration range of heart fatty acid binding protein was 1.0-100.Ong/mL,and the minimum detection limit was 0.12ng/mL.4.Based on the internal filtration effect fluorescence quenching magnetic effect separation immunoassay detection system,the linear concentration range of cardiac troponin I was 0.2-30.0ng/mL,and the minimum detection limit was 0.03ng/mL.5.The optical fiber sensing technology and microsequence injection are used to realize the real-time on-line concentration detection of ultraviolet and fluorescence spectrum.The results were not significantly different from those measured by physicochemical benzyl and vitamin B2(p>0.05).Conclusion:Background fluorescence quenching immune chromatography is a combination of spectral analysis and immunoassay technology,strong specificity,rapid detection,filtering effect within the fluorescence quenching of magnetic separation of immunoassay detection is a combination of fluorescence quenching,immune analysis,spectrum analysis magnetic separation technology,high sensitivity,strong specificity.Both methods can achieve qualitative and quantitative detection of two kinds of cardiac markers,and provide a new technique for clinical application.It can be popularized in communities and community medical institutions.