The Expression Level Of MCP-1?TIMP-1 In The Early Renal Fibrosis And Its Clinical Significance

Objective: Through the preparation of 5/6 nephrectomy rat model,to observe the relationship between-1 tissue inhibitor of metalloproteinase-1(TIMP-1)and monocyte chemoattractant protein-1(MCP-1)and early renal fibrosis in rats,and to explore the feasibility of TIMP-1 and MCP-1 as a biological marker for early renal fibrosis.Methods: Clean male rats were randomly divided into model group(establishment of 5/6 nephrectomy rat model)and control group(without nephrectomy,except for renal capsule).The pathological changes of renal tissue in renal failure rats were observed and observed by HE,Masson and PASM staining.The expression levels of hyaluronic acid,laminin,III procollagen and type IV collagen in two groups of rats were detected by enzyme-linked immunosorbent assay(ELISA).The expression level of kidney tissue were detected by fluorescence quantitative PCR method in two groups of rats in MCP-1 and TIMP-1m RNA,combined with the level of renal pathological changes and biochemical parameters such as serum creatinine,urea nitrogen,uric acid,C reactive protein,24 hour urinary protein quantitative assessment,MCP-1 and TIMP-1 in renal fibrosis in early.Results: After successful modeling,the pathological changes of kidney failure rats in model group were positively correlated with serum creatinine,urea nitrogen,uric acid,C reactive protein and 24 hour urine protein level at fourth,eighth,twelfth weeks after modeling.Glomerular score,renal failure rat model group the interstitial score,serum creatinine and blood urea nitrogen,uric acid,C reactive protein,24 hours urineprotein were higher than the control group,the difference was statistically significant(P<0.05);model group rats after 4,8 and 12 weeks,the expression of MCP-1 in kidney tissues of rats increased gradually,the expression of TIMP-1 decreased gradually,the expression level of two with early renal fibrosis process.Conclusion: The expression of MCP-1 and TIMP-1 in renal tissue of model group is consistent with pathological changes of blood biochemistry and renal fibrosis.It can be used as a potential biomarker for early renal fibrosis.