The Change Of Peripheral Blood T Cell Subsets And Influence Of Metformin On Th17/Treg In Patients With Primary Sjogren's Syndrome

Objective:1.To research the changes of peripheral blood lymphocyte subsets,CD4+T cell subsets and their correlation in patients with PSS;2.The influence of metformin on Th17/Treg in peripheral blood of patients with BD.Methods:This study included a total of 80 patients with PSS,including 9 males and 71 females,aged 18-65 years,with an average of 37.85±12.43 years.The average duration of disease was 2.45±4.97 years.80 cases of gender-and age-matched normal controls were included.The group detailed records of clinical data and laboratory indicators related to PSS patients.All subjects were tested for lymphocyte subsets and CD4+ T cell subsets by flow cytometry.Forty-nine patients with PSS received metformin treatment(250 mg twice daily)for 6 weeks.After the end of the treatment period,the above cells were again tested.ALL the experimental data were analyzed by SPSS17.0 software.Results:1.In the comparison of subpopulations of CD4+ cells,absolute counts of Treg cells in the PSS group were significantly decreased(P=0.000),and the ratio of Th17/Treg cells was significantly higher(P=0.003).There was no statistically significant difference in the number of remaining cells such as Th1,Th2,Th17 and Th1/Th2 between the two groups.2.Absolute counts of Treg cells in PSS patients were negatively correlated with ESR(r=-0.493,P<0.05).3.The number of Treg cells in the traditional treatment group and the non-drug group was lower than that in the normal control group,and there was statistical significance(P<0.000),and the Th17/Treg ratio was higher than the normal control group(P<0.05)..4.ESR was significantly lower after metformin treatment than before metformin treatment(P=0.021).Metformin treatment group Treg cells were higher than those without metformin treatment(p=0.000),and after treatment with metformin,Th17/Treg was lower than metformin treatment(p=0.013),and the remaining CD4+ T cell subsets were not Significant differences between the two groups..5.No significant difference in CD4+ T cell subsets after treatment with metformin and healthy control.Conclusion:1.The Treg cells in peripheral blood of PSS patients decreased,the ratio of Th17/Treg cells increased,there was an imbalance of Th17/Treg cells,and the activity of Treg cells was correlated;Treg cells were negatively correlated with PSS disease activity2.Metformin can promote Treg cell proliferation,restore Th17/Treg cell balance,restore ESR.3.Traditional immunosuppressive agents have no significant effect on the balance of Treg cells and Th17/Treg.