| Chinese medicine decoction pieces which after processing have difference in effectiveness,medicinal properties and toxicity.So should not be confused.And after processing the character change a lot,it’s difficult to identify.Their infrared spectrum is a variety of chemical composition of the comprehensive reflection,can be used to identify Chinese medicine decoction piece quickly.In this study,the following four respects were researched by combining FTIR with pattern recognition models and statistics:Firstly,Mid-Infrared spectroscopy combined with SIMCA to distinguish Radix curcumae(Yujin)and Rhizoma zedoariae(Ezhu).Collected and tested 18 samples of Yujin,18 samples of Ezhu.They have 12 peaks of their 1D-FTIR at the same locations,which at 2929,1648~1655,1416,1375,1238,1156,1081,1022,929,850,764 and 707 cm-1.The 1D-FTIR spectra between the two herbs were different.The peaks at 1515 cm-1(Absorbance was 0.34),were obvious in Yujin,while they were not observed in Ezhu.They have 14 peaks of their SD-FTIR at the same locations,which at 1640,1516,1468,1369,1204,1159,1107,1080,1057,1020,990,929,893 和 846 cm-1;Yujin and Ezhu have the same peaks of 3400,1640 和 1516 cm-1,which are the characteristic peaks of curcumin,but the intension of curcumin characteristic peak of Ezhu was much higher than Yujin,it shows that Curcumin content in Ezhu was higher than Yujin.1D-FTIR of 13 EZ and 13 YJ were selected to build SIMCA clustering analysis method,then the rest of YJ and EZ samples vertify tne model,the rate of identification and rejection is 100%.The 1D-FTIR and SD-FTIR had their characteristic peaks.It shows that SIMCA of FTIR can be used to identify this 2 kinds of decoction piece.Secondly,Ttri-step IR analyse Radix achyranthis bidentatae(Niuxi)and Radix achyranthis bidentatae(Chuanniuxi).Collected and tested 12 Niuxi and 10 Chuanniuxi by 1D,SD,2D-COS FTIR spectroscopy.It’s difficult to identify Niuxi and Chuanniuxi,for their 1D-FTIR were exactly similar.There are differences of their SD-FTIR,Niuxi has the peak of 1671 cm-1,while the Chuanniuxi has the peak of 1620 cm-1.Niuxi and Chuanniuxi have the same peaks of 1317,781 and 662 cm-1,which are the characteristic peaks of calcium oxalate,but the intension of calcium oxalate characteristic peak of Chuanniuxi was much higher than Niuxi,it shows that calcium oxalate content in Chuanniuxi was higher than Niuxi.The number,position and interrelation of auto-peaks on the 2D-FTIR spectra were different between the two herbs.There were 5 auto-peaks in Niuxi,while 4 auto-peaks in Chuanniuxi in the range of 850~1150 cm-1.There were 5 auto-peaks in Niuxi,while 6 auto-peaks in Chuanniuxi in the range of 1150~1500 cm-1.Based on that,Niuxi and Chuanniuxi can be quickly differentiated.Thirdly,Mid-Infrared spectroscopy combined with principal component analysis(PCA)to distinguish different decoction piece of Notopterygium incisum(Qianghuo)and N.forbesii(Kuanyeqiagnhuo).4 Qianghuo and 8 Kuanyeqiagnhuo samples were collected and tested.The 1D-FTIR spectra between the two herbs were different.The peaks at 1739(Absorbance was 0.39),1428(0.46),1076(0.91),863(0.03)and 764(0.08)cm-1 were obvious in Qianghuo,while they were not observed in Kuanyeqianghuo.Meanwhile,the peaks at 1719(0.34),1607(0.50),1444(0.41),823(0.06)and 775(0.07)cm-1 were obvious in Kuanyeqianghuo,but they are not found in Qianghuo.In the PCA dendrogram,Qianghuo and Kuanyeqianghuo were distributed in different areas.Comparing the SD-FTIR spectra found that their spectra were obviously different.The peaks located at 1747,1468,1159,1078 and 988 cm-1 in Qianghuo were significantly stronger than those in Kuanyeqianghuo,while the peaks located at 1627,1605,1568,1512 and 1269 cm-1 in Kuanyeqianghuo were significantly stronger than those in Qianghuo.Moreover,the number,position and interrelation of auto-peaks on the 2D-FTIR spectra were different between the two herbs.There were 13 auto-peaks in Qianghuo,while 9 auto-peaks in Kuanyeqianghuo in the range of 850~1500 cm-1.It shows that the two medicinal raw materials of Qianghuo and Kuanyeqianghuo can be quickly differentiated.Fourth,Mid-Infrared spectrum fingerprint to identify Carthamus tinctorious(Honghua)and dyed Carthami Flos(Ransehonghua).26 samples of Honghua、3 Ransehonghua and 12 homemade Ransehonghua samples were collected and tested by Fourier Transform Infrared(FTIR).There are considerable differences in their position 、number and strength of the characteristic peak between the Honghua and Ransehonghua,They only have 3 peaks of their 1D-FTIR at the same locations,which at 1635,1515 and 778 cm-1.The 1D-FTIR spectra between them were different.The peaks at 1737,1416,1243,1067 and 833cm-1 were obvious in honghua,while they were not observed in Ransehonghua.And the peaks at 1430,1079,634 and 610 cm-1 were obvious in Ransehonghua,while they were not observed in honghua.The 1D-FTIR is vary with homemade Ransehonghua with different addition amount of colouring agent.Building the average spectra of the 20 Honghua as the spectrum fingerprint of Honghua,and the limited level(Mean-3σ)to identify true or false Honghua by correlation coefficient of 1D-IR was confirmed.Then,6 Honghua and 3 Ransehonghua samples were used as validation samples to evaluate the identification efficacy.The result shows that Honghua can be identified from Ransehonghua for the established IR fingerprint.The limited level(0.9882)is exact.It is concluded that the FTIR fingerprint developed can be rapidly and accurately identify Chinese medicine decoction piece.Fifth,The standard of research identification of five kinds of native sichuan decoction piece by infrared spectroscopy: More than 10 Chinese medicine decoction piece samples of different bitchs from different maker were collected and tested by Fourier Transform Infrared(FTIR),then analyse the correlation between the samples and their infrared spectrum,located the range of these decoction pieces characteristic finger-print,established identification method and standard of these decoction pieces. |
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