Isolation And Identification Of Chemical Constituents From Camptosorus Sibiricus

ObjectiveCamptosorus sibiricus is a herbal medicine widely distributed in the North of China,which has good therapy effect on vascular inflammation,liver cancer,and traumatism as a famous folk medicine.However,the bioactivities or pharmacological effects of the C.sibiricus remain to be elucidated.Thus,it is necessary to make systematic investigation on chemical constituents from C.sibiricus.Methods and ConclusionUsing various column chromatographic technologies,(silica gel,Sephadex LH-20 and ODS column chromatography)as well as HPLC,23 compounds have been isolated from the water extract of C.sibiricus.By physical and chemical properties as well as spectroscopic methods,they were elucidated to 3?,25?,30,23,24-pentahydroxycycloartane-3-O-?-D-glucopyranosyl-25-O-?-D-glucopyranosyl-30-O-?-D-glucopyranoside(CS-1),3?,23,24,30-tetrahydroxycycloartane-25(26)-en-3-O-?-D-glucopyranosyl-(1?4)-?-D-glucopyranosyl-(1?2)-?-D-glucopyranoside(CS-2),3,8,23,24,30-tetrahydroxycycloartane-25(26)-en-3-O-?-D-glucopyranosyl-(1?2)-?-D-glucopyranosyl-(1?2)-?-D-glucopyranosyl-(1?4)-?-D-glucopyranoside(CS-3),kaempferol-3-O-?-D-glucopyranoside(CS-4),kaempferol-3,7-diglucoside(CS-5),aempferol-3-O-?-D-glucoside-7-O-?-L-rh amnoside(CS-6),kaempferol-3-O-?-D-glucopyranosyl-(1?2)-?-D-glucopyranoside(CS-7),camsibriside B(CS-8),camsibriside A(CS-9),(7E)-9-Hydroxymegastigma-4,7-dien-3-on-9-0-?-D-glucoside(CS-10),Bridelionoside F(CS-11),(3R,5S,6S,7E,9S)-megastigman-7-ene-3,5,6,9-tetrol9-O-?-D-glucopyranoside(CS-12),(6S,7E,9R)-roseoside(CS-13),(3S,5S,6R,9R)-3-hydroxy-5,6-epoxy-?-ionol-9-0-?-glucopyranoside(CS-14),blumenol A(CS-15),(3R,6R,7E,9R)-3,9-Di hydroxy-4,7-megastigmadiene(CS-16),megastigmane-7-en-3,6-epoxy-5,9-diol9R-O-?-D-glucopyranoside(CS-17),D-mannitol(CS-18),elaphoside A(CS-19),ptetatoside A(CS-20),n-butyl-a-?-D-fructofuranoside(CS-21),Dibutylphthalate(CS-22),D(+)-Sucrose(CS-23).ConclusionAmong them,CS-1,CS-2 and CS-3 are new compounds;CS-10,CS-11,CS-12,CS-13,CS-14,CS-15,CS-16,CS-17,CS-19,CS-20,CS-21 and CS-22 were isolated from C.sibiricus for the first time.