The Enzymatic Hydrolysis Of Ginseng And Its Active Research

Ginseng,entitled "King of Herbs",is the root and rhizome of Panax ginseng C.A.Meyer,which is the treasure in rare medicinal herbs.Ginseng has varieties of ingredients,such as ginsenoside,polysaccharide,protein,polypeptide,amino acids,fatty acids flavonoids,polyacetylene alcohol,volatile oil,vitamin,trace elements.Zheng Yinan et al found that Argininyl-fructoyl-glucose(AFG)and Argininyl-glucose(AF)in red ginseng,and had pointed out that they are Maillard reaction products of argininyl and glucose or maltose occur,further study that AF could enhance cellular immune function,improve peripheral circulation,improve the symptoms of patients with diabetes mellitus etc.Chang Cheng study found that AFG could reducing blood lipid of diabetic Mice Induced by Alloxan and improve its related disease.Yukio Suzuki et al through establishment the red ginseng processing model to study the relationship with AF?AFG between the red ginseng processing browning,and point out that the arginine and reducing sugars(glucose and maltose)increases could accererate between the browning degree.Arginine content is higher in ginseng,suggesting that if add sugars(glucose or maltose)content in ginseng,could produce more AF?AFG in ginseng.In the paper,based on the above theory,we used cellulose and amylase combined enzyme,could increase the reduction of glucose and maltose content in ginseng,increase the content AF?AFG in ginseng.In the word,by the addition cellulose and ?-amylase in ginseng to preparated a kind of ginseng-enzymatic hydrolysis of ginseng,compare the single factor,choose the best suitable for the two kinds of enzyme activity,respectively is pH 5.2,temperature 55 ?,time 2h,and pH5.5,temperature 55 ?,time 24h.Analysis the arginine derivatives(AF?AFG)and the content of total ginsenoside in the ginseng and enzyme ginseng.We founded that after enzymatic hydrolysis ginseng the glucose and maltose were increased 3 times,4 times;the ginsenosides increased from 16.7 mg/g to 19.3 mg/g;the content of AF?AFG were increased by 2.3%,84.5%.In this paper,compare two kinds of ginseng treatment on CCL4 induced acute liver injury anti-fatigue and oral glucose tolerance of normal mice,the enzynolysis ginseng is better than ginseng on acute liver injury of normal mice;the enzymolysis ginseng is significant than ginseng;enzymolysis ginseng is stronger than ginseng on the normal mice of oral glucose tolerance.