The Clinical Application Of Quantitative Enzyme Immunoassay Using Recombinent TrxTSHRn Protein As Antigen

Objective:To measured the TRAb content and the positive rate in the serum of AITD patients by quantitative Enzyme immunoassay using Recombinent TrxTSHRc Protein as antigen,in which could be used in clinical diagnosis,differential diagnosis,treatment monitoring and prognosis for AITD patients,especially GD patients.Methods:136 cases of thyroid disease were divided into eight groups:the initial treatment of hyperthyroidism group,remission group,the hyperthyroidism drug-induced hypothyroidism group,Hashimoto's hypothyroidism group,thyroid adenoma group,and the group which thyroid function is normal,but the thyroid auto-antibodies is positive.The control group was 27 cases of healthy volunteers.By determing the thyroid function FT3,FT4,T3,T4,TSH and thyroid auto-antibodies including TGAb,TPOAb,TRAb levels,we carried out inter-group analysis and compared TRAb positive rate of thyroid disease and its significance.Results:1.TRAb(TSAb-based)concentration comparison? The TRAb concentration in the initial treatment of hyperthyroidism group was significantly higher than the other groups(P<0.01);? GD initial group was significantly higher than the GDremission group;? The TRAb concentration in the initial treatment group,remission group,the hyperthyroidism drug-induced hypothyroidism group was significantly higher than the control group,P<0.05;? There was no significant difference in the thyroid adenoma group,the group which thyroid function is normal,but the thyroid auto-antibodies is positive and the control group.2.TRAb(TSAb-based)positive rate comparison? The positive rate in the initial treatment of hyperthyroidism group was 84%,it was significantly higher than the remission group(P<0.01);?The positive rate of TRAb in each group with GD and Hashimoto's hypothyroidism group,the GD drug-induced hypothyroidism group was significantly higher than the control group,P<0.05;(3)There was no significant difference in the thyroid adenoma group,the group which thyroid function is normal,but the thyroid auto-antibodies is positive and the control group(P>0.05).Conclusion:TRAb has very important clinical significance for the occurrence,evolution,Outcome,prognosis and recurrence of autoimmune thyroid disease.