Extraction And Identification Of The Glycyrrhizin Departed From Different Parts Of Liquorice And Biotransformation

In this paper,the extraction of glycyrrhizin from different parts of glycyrrhiza was studied,and the optimum conditions of industrial water extraction were determined.Simultaneously,the components of extractive which isolated from different parts of liquorice were determined by high performance liquid chromatography.Furthermore,the culture which can transform glycyrrhizin into monoglucuronide-glycyrrhizin was screen out successfully.The optimum conditions of industrial extraction of glycyrrhizin by water was extracting with water(about 10 times volume)for 1.5 h at 100?;the extraction was repeated for 4 times.Then the glycyrrhizin was extracted from liquorice roots,stems,leaves,seeds by the optimum conditions.Simultaneously,the composition of different extractives was identified by HPLC.The results show that the content of glycyrrhizin in Liquorice root is the highest;but glycyrrhizin was not recognized in the leaves and seeds.The yield of glycyrrhizin from liquorice roots was 1 1.3%.These results provided scientific guides for industrialization.In order to screen out the culture which have the ability to produce Glycyrrhizinate beta-glucuronidase from cultures existing in our lab,glycyrrhiza was used as inducer,and the Absidia.sp.GC3 strain was successful screen out.The crude enzyme from Absidia.sp.GC3 strain could hydrolyze glycyrrhizin into monoglucuronide-glycyrrhizin.In addition,the optimal inducer,the optimal dose of the inducer,optimal culture time,optimal enzyme reaction time was studied,respectively.The optimal inducer was glycyrrhiza;the optimal dose of the inducer was 20%;the optimal culture time was 7 d;the optimal enzyme reaction time was 12 h.Simultaneously,Sediment of Ammonium Sulfate method was used to extract enzyme from pork liver,sheep liver,beef liver.we can found that the activity of Glycyrrhizinate beta-glucuronidase in pork liver was the highest and the optimal enzyme reaction time was about 6 h.